The impact of Srebf2 deletion on the transcriptional phenotype of visceral adipose tissue (VAT) regulatory T (Treg) cells
RNA-seq (gene expression) · mouse · 11 samples
- What it is
- RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
- Organism
- Mus musculus (mouse)
- Samples
- 11 samples: 3 × Spl.KO vs 3 × VAT.WT vs 3 × VAT.KO vs 2 × Spl.WTGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- Obesity reshapes regulatory T cells in the visceral adipose tissue by disrupting cellular cholesterol homeostasisScience immunology · 2025
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE243021_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.
Also filed as BioProject PRJNA1015733 and SRA study SRP460003. Searching any of these in the dataset finder brings you back here.
Supports a between-group comparison across 11 samples.
How we read this: 4 replicated groups read from 11 sample titles; they account for 11 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM7777509 Spl.WT.2 Spl.WT
- GSM7777510 Spl.WT.3 Spl.WT
- GSM7777511 Spl.KO.1 Spl.KO
- GSM7777512 Spl.KO.2 Spl.KO
- GSM7777513 Spl.KO.3 Spl.KO
- GSM7777514 VAT.WT.1 VAT.WT
- GSM7777515 VAT.WT.2 VAT.WT
- GSM7777516 VAT.WT.3 VAT.WT
- GSM7777517 VAT.KO.1 VAT.KO
- GSM7777518 VAT.KO.2 VAT.KO
- GSM7777519 VAT.KO.3 VAT.KO
- GSE328134 Adipose tissue inflammation mediated by CCL19 overexpression exacerbates experimental periodontitis via elevated circulating saturated fatty acids and osteopontin in Western-diet-fed mice 36 samples shares vat, adipose
- GSE307127 Immune Receptor Lag3 Deletion Alleviates Neurodegeneration Phenotypes in a Mouse Model of Alzheimer’s Disease 36 samples shares phenotype, deletion
- GSE271769 Impact of severe traumatic brain injury (TBI) on cerebral blood flow and the endothelial cell phenotype 16 samples shares phenotype, impact
- GSE331176 Phagosome-mediated activation of STING by purine and pyrimidine-based bacterial cyclic dinucleotides 380 samples shares vat
- GSE255004 Sensory Flicker Stimulation of 5xFAD Mice Activates Immune Pathways in a Frequency and Time Dependent Manner 176 samples shares vat
- GSE243942 Spatio-temporal X-linked gene reactivation in the mouse germline reveals site-specific retention of epigenetic silencing [RNA-seq] 125 samples shares vat
- GSE297515 Piezo1-dependent activation of stromal cells ignites muscle inflammation in exercise and injury and is associated with inflammaging 90 samples shares vat
- GSE333586 Formylpeptide Receptor Activation Attenuates Renovascular Remodeling in Neurogenic Hypertension (Left Ventricle) 26 samples shares vat
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.