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Bulk RNA-Seq analysis of liver tissues in AAV9-Tnnt2-Cre and AAV9-Tnnt2-Cre-miR122 injected mice

GSE245089 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2025/10/10 Platform GPL30215
Summary
The cardiac troponin T (Tnnt2) promoter is broadly utilized for cardiac specific gene expression, particularly via adeno-associated virus (AAV)-based gene transfer. However, these vectors drive lower-level ectopic gene expression in other tissues, most notably in the liver. Whether the AAV-Tnnt2 vectors remain tissue-specific in applications sensitive to low or transient gene expression, such as gene editing, remains unclear. Inclusion of target sequences for microRNA-122, a highly expressed, liver-specific microRNA, in the AAV transgene’s 3’ untranslated region (3’ UTR) markedly reduced ectopic transgene expression, genome editing and AAV integration in the liver. The heavily used AAV-Tnnt2 system exhibits liver leakiness that severely impairs the cardiac specificity of AAV-based genetic manipulation. This problem can be mitigated via miR122-mediated liver detargeting.
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Direct links to NCBI, no account and no request form: the whole study as GSE245089_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1026924 and SRA study SRP465892. Searching any of these in the dataset finder brings you back here.

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