GEO series

CCR1 and CCR2 co-expression on monocytes is non-redundant and delineates a distinct monocyte subpopulation. [scRNA-Seq]

GSE251647 Mus musculus (mouse) Single-cell RNA-seq 1 samples Submitted 2024/07/10 Platform GPL30172

At a glance

Single-cell RNA-seq · mouse · 1 samples

What it is: Single-cell RNA-seqGene activity measured in thousands of individual cells, so cell types can be told apart.
Organism: Mus musculus (mouse)
Samples: 1 samples
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper: CCR1 and CCR2 Coexpression on Monocytes Is Nonredundant and Delineates a Distinct Monocyte SubpopulationJournal of immunology (Baltimore, Md. : 1950) · 2024

Summary (written by the submitters)

The interactions between chemokines and their receptors, particularly in the context of inflammation, are complex with individual receptors binding multiple ligands and individual ligands interacting with multiple receptors. In addition, there are numerous reports of simultaneous co-expression of multiple inflammatory chemokine receptors on individual inflammatory leukocyte subtypes.

Read the full summary ↓

Overall, this has previously been interpreted as redundancy and proposed as a protective mechanism to ensure that the inflammatory response is robust. In contrast we have hypothesised that the system is not redundant but exquisitely subtle. Our interests relate to the receptors CCR1, CCR2, CCR3 and CCR5 which, together, regulate non-neutrophilic myeloid cell recruitment to inflammatory sites. Here we demonstrate that, whilst most murine monocytes exclusively express CCR2, there is a small subpopulation, which is expanded during inflammation, which co-expresses CCR1 and CCR2. Combinations of transcript and functional analysis demonstrate that this is not redundant expression and that co-expression of CCR1 and CCR2 marks a phenotypically distinct population of monocytes characterised by expression of genes otherwise typically associated with neutrophils. Single cell RNA sequencing confirms this as a monodisperse population of atypical monocytes. This monocytic population has been previously described as having immunosuppressive activity. Overall, our data confirm combinatorial chemokine receptor expression by a subpopulation of monocytes but demonstrate that this is not redundant expression and marks a discrete monocytic population.

Published in

CCR1 and CCR2 Coexpression on Monocytes Is Nonredundant and Delineates a Distinct Monocyte Subpopulation

Medina-Ruiz L, Bartolini R, Mathie H et al. · Journal of immunology (Baltimore, Md. : 1950) 2024 · PMID 38829123 · doi:10.4049/jimmunol.2400007

This dataset

Open GSE251647 in finder → Find more mouse RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE251647_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: Single-cell studies usually ship their counts as 10x files (matrix.mtx, barcodes.tsv, features.tsv), an .h5 file, or an .rds / .h5ad object that Seurat or Scanpy can open. Look for those among the supplementary files.

Also filed as BioProject PRJNA1055015 and SRA study SRP479131. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 38829123) ↗

Samples in this study

The sample list for this study is not cached yet. Press Load samples and it will be fetched from NCBI.

+ 1 more — browse all 1 samples with per-sample file links →

Datasets on a similar topic

GSE251648 CCR1 and CCR2 co-expression on monocytes is nonredundant and delineates a distinct monocyte subpopulation. 20 samples shares ccr1, ccr2, co-expression, subpopulation, delineates, monocytes
GSE251646 CCR1 and CCR2 co-expression on monocytes is nonredundant and delineates a distinct monocyte subpopulation. [RNA-Seq] 19 samples shares ccr1, ccr2, co-expression, subpopulation, delineates, monocytes
GSE301118 Targeting Ccr1, Ccr3, Ccr2 and Ccr5 in H3.1K27 Mutant Diffuse Midline Gliomas (DMGs). 6 samples shares ccr1, ccr2
GSE277303 Inducible CCR2+ nonclassical monocytes mediate the regression of cancer metastasis 42 samples shares ccr2, monocytes
GSE232461 Long term effects of CCR2+ myeloid cell depletion on islet cell populations identified by scRNA-seq 4 samples shares ccr2, scrna-seq
GSE277155 CCR2 limits inflammatory functions of CD8 TRM cells that impair recognition memory during recovery from WNV encephalitis [scRNA-seq] 4 samples shares ccr2, scrna-seq
GSE249242 B cells mediate lung ischemia-reperfusion injury by recruitment of CCR2+-classical monocytes via synergistic BCR-TLR4 signaling 3 samples shares ccr2, monocytes
GSE122765 Transcriptome analysis of pulmonary CCR2+ inflammatory monocytes challenged with Cryptococcus neoformans 3 samples shares ccr2, monocytes

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