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Transcriptome sequencing across a prostate cancer cohort identifies PCAT-1, an unannotated lincRNA implicated in disease progression (RNA-Seq data)

GSE25183 Homo sapiens Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing 58 samples Submitted 2011/08/02 Platform GPL9115Platform GPL9052
Summary
Noncoding RNAs (ncRNAs) are emerging as key molecules in human cancer, with the potential to serve as novel markers of disease and to reveal uncharacterized aspects of tumor biology. Here we discover 121 unannotated prostate cancer–associated ncRNA transcripts (PCATs) by ab initio assembly of high-throughput sequencing of polyA+ RNA (RNA-Seq) from a cohort of 102 prostate tissues and cells lines. We characterized one ncRNA, PCAT-1, as a prostate-specific regulator of cell proliferation and show that it is a target of the polycomb repressive complex 2 (PRC2). We further found that patterns of PCAT-1 and PRC2 expression stratified patient tissues into molecular subtypes distinguished by expression signatures of PCAT-1–repressed target genes. Taken together, our findings suggest that PCAT-1 is a transcriptional repressor implicated in a subset of prostate cancer patients. These findings establish the utility of RNA-Seq to identify disease-associated ncRNAs that may improve the stratification of cancer subtypes.
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Direct links to NCBI, no account and no request form: the whole study as GSE25183_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 58 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA153913 and SRA study SRP004637. Searching any of these in the dataset finder brings you back here.

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