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Cysteine Rich Protein 2 is a copper-responsive regulator of skeletal muscle differentiation. [RNA-Seq]

GSE252158 Mus musculus Expression profiling by high throughput sequencing 9 samples Submitted 2024/05/30 Platform GPL30172
Summary
Copper (Cu) is an essential trace element for diverse biological reactions such as respiration, neurotransmitter synthesis, oxidative stress and transcriptional regulation. If Cu homeostasis is disrupted, several pathological conditions can develop, leading to alterations of neuronal, cognitive, and muscular development. Known Cu+-binding transcription factors in mammalian cells are Atox1, Mtf1 and Sp1. We identified Crip2 as a novel Cu+-responsive transcriptional regulator that is required for the differentiation of primary myoblasts derived from mouse satellite cells. Functional characterization of CRISPR/Cas9-mediated deletion of Crip2 showed that myoblasts fail to differentiate and manifest a decrease in expression of the differentiation markers Myogenin and Myosin heavy chain. RNA-seq and CUT&RUN analysis were performed to understand the effect on Crip2 in the regulation of gene expression in proliferating and differentiating primary myoblasts stimulated with Cu.
Published in
Cysteine Rich Intestinal Protein 2 is a copper-responsive regulator of skeletal muscle differentiation
Verdejo-Torres O, Klein DC, Novoa-Aponte L et al. · bioRxiv : the preprint server for biology 2024 · PMID 38746126 · doi:10.1101/2024.05.03.592485
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Also filed as BioProject PRJNA1057942 and SRA study SRP480342. Searching any of these in the dataset finder brings you back here.

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