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Single-nuclei characterization of oxycodone's effects on dorsal peduncular nucleus

GSE260687 Mus musculus Expression profiling by high throughput sequencing 4 samples Submitted 2024/06/07 Platform GPL24247
Summary
In addition to their intrinsic rewarding properties. opioids can also evoke aversive reactions that protect against misuse. Cellular mechanisms that govern the interplay between opioid reward and aversion are poorly understood. We used whole-brain activity mapping to show that neurons in the dorsal peduncular nucleus (DPn) are highly responsive to the opioid oxycodone. Connectomic profiling revealed that DPn neurons innervate the parabrachial nucleus (PBn). Spatial and single-nuclei transcriptomics resolved a unique population of PBn-projecting pyramidal neurons restricted to the DPn that express μ-opioid receptors (μORs). Disrupting μOR signaling in these neurons switched oxycodone from rewarding to aversive and exacerbated the severity of opioid withdrawal. These findings identify DPn neurons as key substrates for the abuse liability of opioids.
Published in
A master regulator of opioid reward in the ventral prefrontal cortex
Smith ACW, Ghoshal S, Centanni SW et al. · Science (New York, N.Y.) 2024 · PMID 38843332 · doi:10.1126/science.adn0886
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Also filed as BioProject PRJNA1082687 and SRA study SRP492826. Searching any of these in the dataset finder brings you back here.

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