Microglial NLRP3-gasdermin D activation impairs blood-brain barrier integrity through interleukin-1β-independent neutrophil chemotaxis upon peripheral inflammation
RNA-seq (gene expression) · mouse · 10 samples
- What it is
- RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
- Organism
- Mus musculus (mouse)
- Samples
- 10 samples: 5 conditions, mostly in duplicateGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- Microglial NLRP3-gasdermin D activation impairs blood-brain barrier integrity through interleukin-1β-independent neutrophil chemotaxis upon peripheral inflammation in miceNature communications · 2025
Read the full summary ↓
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE263094_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.
Also filed as BioProject PRJNA1095828 and SRA study SRP499486. Searching any of these in the dataset finder brings you back here.
Supports a between-group comparison across 10 samples.
How we read this: 5 replicated groups read from 10 sample titles; they account for 10 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM8185728 Brain, WT PBS, replicate 1 Brain, WT PBS,
- GSM8185729 Brain, WT LPS x1, replicate 1 Brain, WT LPS x1,
- GSM8185730 Brain, WT LPS x2, replicate 1 Brain, WT LPS x2,
- GSM8185731 Brain, KO PBS, replicate 1 Brain, KO PBS,
- GSM8185732 Brain, KO LPS x2, replicate 1 Brain, KO LPS x2,
- GSM8185733 Brain, WT PBS, replicate 2 Brain, WT PBS,
- GSM8185734 Brain, WT LPS x1, replicate 2 Brain, WT LPS x1,
- GSM8185735 Brain, WT LPS x2, replicate 2 Brain, WT LPS x2,
- GSM8185736 Brain, KO PBS, replicate 2 Brain, KO PBS,
- GSM8185737 Brain, KO LPS x2, replicate 2 Brain, KO LPS x2,
- GSE159295 Translation-dependent and independent mRNA decay occur through mutually exclusive pathways that are defined by ribosome density during T Cell activation [RNA-Seq] 105 samples shares independent, activation
- GSE279388 Inactivation of Atp7b copper transporter in the intestinal epithelial cells is associated with altered lipid processing and cell growth machinery that are independent from hepatic copper accumulation and liver histology severity 76 samples shares independent, activation
- GSE300925 Lyn expression in macrophages promotes TLR activation and restricts proliferation in an isoform-independent manner 48 samples shares independent, activation
- GSE319318 Inactivation of CDKN2A-ARF Promotes p53-Independent Remodeling of the PDAC Tumor Microenvironment [RNA-Seq] 20 samples shares independent, activation
- GSE303157 Lcn2 deficiency leads to social impairments independent of maternal immune activation 18 samples shares independent, activation
- GSE284259 A single Citrobacter rodentium infection in Pink1 knockout and wild type mice leads to regional blood-brain-barrier perturbation and glial activation without dopamine neuron axon terminal loss 17 samples shares blood-brain, activation
- GSE319693 TLR9-Mediated Macrophage-ILC Activation Induces Antigen-Independent Antiviral Immune Memory in the Lung 12 samples shares independent, activation
- GSE225367 GPR124 regulates murine CNS angiogenesis and blood-brain barrier formation by an intracellular domain-independent mechanism 12 samples shares blood-brain, independent
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.