GEO series

Cold-inducible brown fat thermogenesis enhances MHC class I expression and antitumor immunity in ovarian cancer.

GSE264447 Mus musculus (mouse) RNA-seq (gene expression) 28 samples Submitted 2025/12/31 Platform GPL24247

At a glance

RNA-seq (gene expression) · mouse · 28 samples

What it is: RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism: Mus musculus (mouse)
Samples: 28 samples: 6 conditions, mostly with about 6 replicates eachGroups are read from the sample names, not from the paper. Check the list below.
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.

Summary (written by the submitters)

Brown adipose tissue (BAT) thermogenesis, induced by cold exposure or β3-adrenergic receptors (β3AR) agonist, decreases tumor glucose availability and arrests tumor progression. However, the effect of BAT activation on tumor metabolic reprogramming, the tumor microenvironment (TME), and the possibility of in combination with immunotherapy has not been defined.

Read the full summary ↓

Here, we found that cold-inducible BAT thermogenesis promoted an anti-Warburg metabolic shift that reshaped TME characterized by accumulation of tumor-infiltrating CD8+ T cells (CD8+ TILs) with effector-like and memory phenotype and proinflammatory macrophages. Consistently, combination treatment with anti-PD-1 and cold exposure or β3AR agonist, strongly inhibited tumor growth or complete regressions across multiple preclinical mouse models. Therefore, BAT thermogenesis by cold exposure or β3aR agonist, highlighting a promising therapeutic approach to increase tumor immunogenicity and improve immunotherapy efficacy.

This dataset

Open GSE264447 in finder → Find more mouse RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE264447_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1102423 and SRA study SRP502931. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗

Study design

6 conditions, mostly with about 6 replicates each

Tumor tissues, Control, ×6 Tumor tissues, Cold, ×6 Tumor cells, Control, ×5 CD8TILs, Cold, ×4 Tumor cells, Cold, ×4 CD8TILs, Control, ×3

Supports a case/control comparison: 10 samples read as cases, 3 as controls.

How we read this: 6 replicated groups read from 28 sample titles; they account for 28 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

GSM8218323 Tumor tissues, Control, rep1 Tumor tissues, Control,
GSM8218324 Tumor tissues, Control, rep2 Tumor tissues, Control,
GSM8218325 Tumor tissues, Control, rep3 Tumor tissues, Control,
GSM8218326 Tumor tissues, Control, rep4 Tumor tissues, Control,
GSM8218327 Tumor tissues, Control, rep5 Tumor tissues, Control,
GSM8218328 Tumor tissues, Control, rep6 Tumor tissues, Control,
GSM8218329 Tumor tissues, Cold, rep1 Tumor tissues, Cold,
GSM8218330 Tumor tissues, Cold, rep2 Tumor tissues, Cold,
GSM8218331 Tumor tissues, Cold, rep3 Tumor tissues, Cold,
GSM8218332 Tumor tissues, Cold, rep4 Tumor tissues, Cold,
GSM8218333 Tumor tissues, Cold, rep5 Tumor tissues, Cold,
GSM8218334 Tumor tissues, Cold, rep6 Tumor tissues, Cold,
GSM8218335 CD8TILs, Control, rep1 CD8TILs, Control,
GSM8218336 CD8TILs, Control, rep2 CD8TILs, Control,
GSM8218337 CD8TILs, Control, rep3 CD8TILs, Control,
GSM8218338 CD8TILs, Cold, rep1 CD8TILs, Cold,
GSM8218339 CD8TILs, Cold, rep2 CD8TILs, Cold,
GSM8218340 CD8TILs, Cold, rep3 CD8TILs, Cold,
GSM8218341 CD8TILs, Cold, rep4 CD8TILs, Cold,
GSM8218342 Tumor cells, Control, rep1 Tumor cells, Control,
GSM8218343 Tumor cells, Control, rep2 Tumor cells, Control,
GSM8218344 Tumor cells, Control, rep3 Tumor cells, Control,
GSM8218345 Tumor cells, Control, rep4 Tumor cells, Control,
GSM8218346 Tumor cells, Control, rep5 Tumor cells, Control,
GSM8218347 Tumor cells, Cold, rep1 Tumor cells, Cold,
GSM8218348 Tumor cells, Cold, rep2 Tumor cells, Cold,
GSM8218349 Tumor cells, Cold, rep3 Tumor cells, Cold,
GSM8218350 Tumor cells, Cold, rep4 Tumor cells, Cold,

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.