Effect of siRNA-mediated GSK3 knock-down on the transcriptional signature of human GM-CSF-dependent monocyte-derived macrophages
RNA-seq (gene expression) · human · 12 samples
- What it is
- RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
- Organism
- Homo sapiens (human)
- Samples
- 12 samples: 12 samples, each with a distinct title — no replicated groupsGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- Reprogramming of GM-CSF-dependent alveolar macrophages through GSK3 activity modulationeLife · 2025
Read the full summary ↓
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE266236_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.
Also filed as BioProject PRJNA1106426 and SRA study SRP504892. Searching any of these in the dataset finder brings you back here.
Any grouping that defines a comparison lives in the sample characteristics, which GEO records separately from the titles.
How we read this: Read from 12 sample titles: 12 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM8242867 siRNA Control-GM-MØ - Donor A
- GSM8242868 siRNA Control-GM-MØ - Donor B
- GSM8242869 siRNA Control GM-MØ - Donor C
- GSM8242870 siRNA GSK3A-GM-MØ - Donor A
- GSM8242871 siRNA GSK3A-GM-MØ - Donor B
- GSM8242872 siRNA GSK3A-GM-MØ - Donor C
- GSM8242873 siRNA GSK3B-GM-MØ - Donor A
- GSM8242874 siRNA GSK3B-GM-MØ - Donor B
- GSM8242875 siRNA GSK3B-GM-MØ - Donor C
- GSM8242876 siRNA GSK3A+GSK3B-GM-MØ - Donor A
- GSM8242877 siRNA GSK3A+GSK3B-GM-MØ - Donor B
- GSM8242878 siRNA GSK3A+GSK3B -GM-MØ - Donor C
- GSE256208 Effect of GSK3 inhibition on the transcriptional signature of human GM-CSF-dependent monocyte-derived macrophages 6 samples shares gsk3, gm-csf-dependent, monocyte-derived, macrophages, signature
- GSE262463 Effect of GSK3 inhibition on the transcriptional signature of human alveolar macrophages 6 samples shares gsk3, macrophages, signature
- GSE256538 Effect of GSK3 inhibition on the transcriptional signature of human monocytes 9 samples shares gsk3, signature
- GSE236156 Mycobacterium tuberculosis induced interferon pathways distinguish responses of Human Alveolar Macrophages from Monocyte-Derived Macrophages 55 samples shares monocyte-derived, macrophages
- GSE262963 Transcriptional profile of human monocyte-derived macrophages stimulated with Interleukin 27 6 samples shares monocyte-derived, macrophages
- GSE232628 Expansion of profibrotic monocyte-derived alveolar macrophages in patients with persistent respiratory symptoms and radiographic abnormalities after COVID-19 46 samples shares monocyte-derived, macrophages
- GSE335752 Characterization of human M2-like (TGFbeta/IL10) vs M0 monocyte-derived macrophages 36 samples shares monocyte-derived, macrophages
- GSE316027 β-hydroxybutyrate-driven histone lysine β-hydroxybutyrylation promotes the immunostimulatory activity of monocyte-derived macrophages in rectal cancer 32 samples shares monocyte-derived, macrophages
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.