GEO series

SGLT2 inhibitor upregulates myocardial genes for oxidative phosphorylation and fatty acid metabolism in Gαq mice (genotype x ERTU)

GSE268984 Mus musculus (mouse) RNA-seq (gene expression) 18 samples Submitted 2025/04/30 Platform GPL30172

At a glance

RNA-seq (gene expression) · mouse · 18 samples

What it is: RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism: Mus musculus (mouse)
Samples: 18 samples: 5 × Gαq CD vs 5 × WT ERTU vs 4 × WT CD vs 4 × Gαq ERTUGroups are read from the sample names, not from the paper. Check the list below.
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper: SGLT2 inhibitor upregulates myocardial genes for oxidative phosphorylation and fatty acid metabolism in Gαq-miceJournal of molecular and cellular cardiology plus · 2025

Summary (written by the submitters)

Background. Mitochondrial dysfunction with decreased ATP production and increased release of reactive oxygen species (ROS) is a hallmark of the failing heart. Although SGLT2 inhibitors have been shown to improve myocardial metabolism in the failing heart, independent of diabetes, the mechanism of this effect is not clear. Objectives.

Read the full summary ↓

Our goal was to test the effect of the SGLT2 inhibitor ertugliflozin on mitochondrial gene expression and function in myocardium and isolated mitochondria from non-diabetic mice with dilated cardiomyopathy due to cardiac-specific over-expression of Gαq. Methods. Gαq and wild-type (WT) littermates 4 weeks of age were treated for 16 weeks with or without the SGLT2 inhibitor ertugliflozin (ERTU) formulated in the chow (0.5 mg/g chow). Results. From weeks 4 to 20, Gαq mice developed progressive cardiac hypertrophy, dilation, contractile dysfunction, myocyte apoptosis and interstitial fibrosis – all of which were prevented by ERTU treatment. Isolated cardiac mitochondria from Gαq mice had decreased maximal ATP production and increased ROS release - both of which were normalized by ERTU. In isolated beating hearts from Gαq mice, contractile reserve and high energy phosphates measured simultaneously by 31P NMR spectroscopy were decreased - and both were improved by ERTU. In Gαq mice, marked suppression of myocardial gene programs for oxidative phosphorylation and fatty acid metabolism was reversed by ERTU. Conclusions. The SGLT2 inhibitor ERTU corrected the expression of myocardial gene programs for oxidative phosphorylation and fatty acid metabolism, thereby leading to increased production of ATP, decreased release of mitochondrial ROS, and amelioration of the consequences of mitochondrial dysfunction on myocardial structure and function.

Published in

SGLT2 inhibitor upregulates myocardial genes for oxidative phosphorylation and fatty acid metabolism in Gαq-mice

Chambers JM, Croteau D, Pimentel DR et al. · Journal of molecular and cellular cardiology plus 2025 · PMID 40291834 · doi:10.1016/j.jmccpl.2025.100296

This dataset

Open GSE268984 in finder → Find more mouse RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE268984_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1119559 and SRA study SRP511481. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 40291834) ↗

Study design

5 × Gαq CD vs 5 × WT ERTU vs 4 × WT CD vs 4 × Gαq ERTU

Supports a between-group comparison across 18 samples.

How we read this: 4 replicated groups read from 18 sample titles; they account for 18 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

GSM8304088 WT CD 1 WT CD
GSM8304089 WT CD 2 WT CD
GSM8304090 WT CD 3 WT CD
GSM8304091 WT CD 4 WT CD
GSM8304092 Gαq CD 1 Gαq CD
GSM8304093 Gαq CD 2 Gαq CD
GSM8304094 Gαq CD 3 Gαq CD
GSM8304095 Gαq CD 4 Gαq CD
GSM8304096 Gαq CD 5 Gαq CD
GSM8304097 WT ERTU 1 WT ERTU
GSM8304098 WT ERTU 2 WT ERTU
GSM8304099 WT ERTU 3 WT ERTU
GSM8304100 WT ERTU 4 WT ERTU
GSM8304101 WT ERTU 5 WT ERTU
GSM8304102 Gαq ERTU 1 Gαq ERTU
GSM8304103 Gαq ERTU 2 Gαq ERTU
GSM8304104 Gαq ERTU 3 Gαq ERTU
GSM8304105 Gαq ERTU 4 Gαq ERTU

Datasets on a similar topic

GSE276289 SGLT2 inhibitor upregulates myocardial genes for oxidative phosphorylation and fatty acid metabolism in Gαq mice (genotype x timepoint) 16 samples shares sglt2, phosphorylation, upregulates, myocardial, inhibitor, oxidative, genotype
GSE276290 SGLT2 inhibitor upregulates myocardial genes for oxidative phosphorylation and fatty acid metabolism in Gαq mice 34 samples shares sglt2, phosphorylation, upregulates, myocardial, inhibitor, oxidative
GSE270093 The SGLT2 Inhibitor Empagliflozin Enhances Metabolic Efficiency and Ameliorates Left Ventricular Hypertrophy in Myosin R403Q Mutant Hypertrophic Cardiomyopathy Mice 21 samples shares sglt2, inhibitor
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GSE293811 Heterozygous Kmt2d loss diminishes enhancers to render medulloblastoma cells vulnerable to combinatory inhibition of lysine demethylation and oxidative phosphorylation [RNA-seq] 10 samples shares phosphorylation, oxidative
GSE282799 IL-1 protects from fatal systemic candidiasis by inhibition of oxidative phosphorylation and hypoxia 10 samples shares phosphorylation, oxidative
GSE248458 Heterozygous Kmt2d loss diminishes enhancers to render medulloblastoma cells vulnerable to combinatory inhibition of lysine demethylation and oxidative phosphorylation 25 samples shares phosphorylation, oxidative
GSE202079 Short term perturbation of mouse embryonic stem cells with morphogens and small molecule compounds 208 samples shares ertu

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.