← BioTransfer GEO Dataset Finder
GEO series

Glucagon receptor agonism and treatment withdrawal effect on liver transcriptomes of diet-induced obese mice

GSE269328 Mus musculus (mouse) RNA-seq (gene expression) 24 samples Submitted 2025/12/30 Platform GPL24247
At a glance

RNA-seq (gene expression) · mouse · 24 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
24 samples: 8 × DIO, 4 d vehicle treatment, 22 h after last injection, vs 8 × DIO, 4 d IUB288 treatment, 4 h after last injection, vs 8 × DIO, 4 d IUB288 treatment, 22 h after last injection,Groups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Summary (written by the submitters)
Glucagon is historically viewed as the main counter-regulatory hormone to insulin in glucose homeostasis. Although chronic glucagon receptor (GCGR) agonism induces hyperglycemia and glucose intolerance, acutely it also improves insulin secretion and action. Surprisingly, we found chronic treatment with the selective, long-acting GCGR agonist, IUB288, improved glycemia 4h after the last injection and hyperglycemia after 12h, compared to vehicle-treated controls, suggesting chronic GCGR agonism is not exclusively hyperglycemic.
Read the full summary ↓
These periods of glycemic improvement were associated with enhanced AKT phosphorylation, despite control-like insulin levels, and could not be replicated by short acting GCGR agonists. Surprisingly, 4d IUB288 treatment in diet-induced obese (DIO) mice reduced ad libitum glycemia for a longer duration than observed in chow IUB288-treated mice. IUB288 treatment also increased glucose infusion rate, suppression of endogenous glucose production, and AKT phosphorylation during hyperinsulinemic-euglycemic clamps.
This dataset
Open GSE269328 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE269328_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1121225 and SRA study SRP512410. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗
Study design
8 × DIO, 4 d vehicle treatment, 22 h after last injection, vs 8 × DIO, 4 d IUB288 treatment, 4 h after last injection, vs 8 × DIO, 4 d IUB288 treatment, 22 h after last injection,

Supports a between-group comparison across 24 samples.

How we read this: 3 replicated groups read from 24 sample titles; they account for 24 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

Search all mouse RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.