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Satellite glial contact enhances differentiation and maturation of human induced sensory neurons

GSE272868 Rattus norvegicus (rat) RNA-seq (gene expression) 14 samples Submitted 2024/07/24 Platform GPL27452Platform GPL24676Platform GPL25947
At a glance

RNA-seq (gene expression) · rat · 14 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Rattus norvegicus (rat)
Samples
14 samples: 4 × rDRG Glia maintained in base glia media vs 4 × Induced human sensory neurons alone vs 3 × iSN-rDRG coculture vs 3 × rDRG Glia treated with NCATS Maturation MediaGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Satellite glial contact enhances differentiation and maturation of human iPSC-derived sensory neuronsStem cell reports · 2025
Summary (written by the submitters)
Induced sensory neurons (iSNs) generated from pluripotent stem cells are used to model human peripheral neuropathies, however current differentiation protocols produce sensory neurons with an embryonic phenotype. Peripheral glial cells contact sensory neurons early in development and contribute to formation of the canonical pseudounipolar morphology, but these signals are not encompassed in current iSN differentiation protocols.
Read the full summary ↓
Here, we show that terminal differentiation of iSNs in co-culture with rodent Dorsal Root Ganglion satellite glia (rSG) advances their differentiation and maturation. Co-cultured iSNs develop pseudounipolar morphology through contact with rSGs. This transition depends on semaphorin-plexin guidance cues and on glial gap junction signaling. In addition to morphological changes, iSNs terminally differentiated in co-culture exhibit enhanced spontaneous action potential firing, more mature gene expression, and increased susceptibility to paclitaxel induced axonal degeneration. Thus, iSNs differentiated in coculture with rSGs provide a better model for understanding human peripheral neuropathies.
Published in
Satellite glial contact enhances differentiation and maturation of human iPSC-derived sensory neurons
LeBlang CJ, Pazyra-Murphy MF, Silagi ES et al. · Stem cell reports 2025 · PMID 40972585 · doi:10.1016/j.stemcr.2025.102639
This dataset
Open GSE272868 in finder → Find more RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE272868_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1139153 and SRA study SRP521615. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 40972585) ↗
Study design
4 × rDRG Glia maintained in base glia media vs 4 × Induced human sensory neurons alone vs 3 × iSN-rDRG coculture vs 3 × rDRG Glia treated with NCATS Maturation Media

Supports a between-group comparison across 14 samples.

How we read this: 4 replicated groups read from 14 sample titles; they account for 14 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
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