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Targeting nucleoporin 214 selectively eliminates leukemia stem cells by inducing ferroptosis [RNA-seq 1}

GSE273642 Mus musculus (mouse) RNA-seq (gene expression) 10 samples Submitted 2025/07/29 Platform GPL24247
At a glance

RNA-seq (gene expression) · mouse · 10 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
10 samples
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Summary (written by the submitters)
Leukemia stem cells (LSCs) give rise to mature leukemia blasts and are the major cause of therapy resistance and relapse in acute myeloid leukemia (AML). We propose a novel strategy to eliminate heterogeneous LSCs but spare hematopoietic stem cells (HSCs) by targeting essential genes that are highly expressed in LSCs than that in HSCs.
Read the full summary ↓
Nucleoporin 214 (NUP214) is determined as a dose-dependently essential gene (DDEG) in LSCs. Homozygous deletion of Nup214 eliminates LSCs and HSCs; however, Nup214 haploinsufficiency impairs LSC maintenance but spares HSCs by selectively inducing ferroptosis of LSCs. Mechanistically, NUP214 locates to chromatin and interacted with Sub1 to inhibit the transcription of heme oxygenase-1 (Hmox1) and arachidonate 15-lipoxygenase (Alox15), thus protecting LSCs against lipid peroxidation. We screened and identified a small molecule 0449-b as a molecular glue that links NUP214 and an NEDD8 E3 ligase, Rbx2, to induce neddylation and degradation of NUP214. 0449-b shows efficacy in selective eradication of LSCs but has a negligible effect on normal hematopoietic stem and progenitor cells. These biological and chemical findings provide a valuable strategy to eliminate LSCs by targeting DDEGs.
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE273642_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1142559 and SRA study SRP523406. Searching any of these in the dataset finder brings you back here.

Samples in this study

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+ 10 more — browse all 10 samples with per-sample file links →

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