GEO series
Adhesion G protein-coupled receptor ADGRG1 promotes protective microglial response in Alzheimer’s disease
GSE273690
Mus musculus (mouse)
RNA-seq (gene expression)
48 samples
Submitted 2025/07/25
Platform GPL24247
At a glance
RNA-seq (gene expression) · mouse · 48 samples
- What it is
- RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
- Organism
- Mus musculus (mouse)
- Samples
- 48 samples: 16 conditions, mostly in triplicateGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- G-protein-coupled receptor ADGRG1 drives a protective microglial state in Alzheimer's disease through MYC activationNeuron · 2025
Summary (written by the submitters)
Germline genetic architecture of Alzheimer's disease (AD) indicates microglial mechanisms of disease susceptibility and outcomes. However, the mechanisms enabling protective microglial responses remain elusive. Here, we investigate the role of microglial ADGRG1, an adhesion G-protein-coupled receptor (aGPCR) specifically expressed in yolk-sac-derived microglia, in AD pathology using the 5xFAD mouse model.
Read the full summary ↓
Transcriptomic analyses reveal that ADGRG1 activates the transcription factor MYC, leading to upregulation of genes involved in homeostasis, phagocytosis, and lysosomal functions, thereby promoting a protective microglial state. We demonstrate that deletion of Adgrg1 in microglia impairs MYC activation, resulting in increased amyloid-beta deposition, exacerbated neuronal loss, and cognitive deficits. Functional assays in mouse models and human embryonic stem cell-derived microglia confirm that ADGRG1 is required for Aβ phagocytosis. These findings uncover a GPCR-mediated pathway that drives a protective microglial state via MYC activation, suggesting potential therapeutic strategies to alleviate AD progression by enhancing microglial functional competence.
Published in
Zhu B, Wangzhou A, Yu D et al.
· Neuron 2025
· PMID 40713954
· doi:10.1016/j.neuron.2025.06.020
This dataset
Download · which file do I need?
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
1 · Just have a look
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
2 · Re-analyse it
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE273690_RAW.tar (it can run to tens of gigabytes).
3 · Start from the raw reads
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.
Also filed as BioProject PRJNA1142856 and SRA study SRP523567. Searching any of these in the dataset finder brings you back here.
Study design
16 conditions, mostly in triplicate
Con male mice, DAPI+, ×4
Con male mice, DAPI+ NeuN-, ×4
Con female mice, DAPI+, ×3
Con female mice, DAPI+ NeuN-, ×3
CKO male mice, DAPI+, ×3
CKO male mice, DAPI+ NeuN-, ×3
CKO female mice, DAPI+, ×3
CKO female mice, DAPI+ NeuN-, ×3
+8 more
Supports a between-group comparison across 48 samples.
How we read this: 16 replicated groups read from 48 sample titles; they account for 48 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
Samples in this study
- GSM8435142 Con male mice, DAPI+, replicate 1 Con male mice, DAPI+,
- GSM8435143 Con male mice, DAPI+, replicate 2 Con male mice, DAPI+,
- GSM8435144 Con male mice, DAPI+, replicate 3 Con male mice, DAPI+,
- GSM8435145 Con male mice, DAPI+ NeuN-, replicate 1 Con male mice, DAPI+ Ne…
- GSM8435146 Con male mice, DAPI+ NeuN-, replicate 2 Con male mice, DAPI+ Ne…
- GSM8435147 Con male mice, DAPI+ NeuN-, replicate 3 Con male mice, DAPI+ Ne…
- GSM8435148 Con female mice, DAPI+, replicate 1 Con female mice, DAPI+,
- GSM8435149 Con female mice, DAPI+, replicate 2 Con female mice, DAPI+,
- GSM8435150 Con female mice, DAPI+, replicate 3 Con female mice, DAPI+,
- GSM8435151 Con female mice, DAPI+ NeuN-, replicate 1 Con female mice, DAPI+ …
- GSM8435152 Con female mice, DAPI+ NeuN-, replicate 2 Con female mice, DAPI+ …
- GSM8435153 Con female mice, DAPI+ NeuN-, replicate 3 Con female mice, DAPI+ …
- GSM8435154 CKO male mice, DAPI+, replicate 1 CKO male mice, DAPI+,
- GSM8435155 CKO male mice, DAPI+, replicate 2 CKO male mice, DAPI+,
- GSM8435156 CKO male mice, DAPI+, replicate 3 CKO male mice, DAPI+,
- GSM8435157 CKO male mice, DAPI+ NeuN-, replicate 1 CKO male mice, DAPI+ Ne…
- GSM8435158 CKO male mice, DAPI+ NeuN-, replicate 2 CKO male mice, DAPI+ Ne…
- GSM8435159 CKO male mice, DAPI+ NeuN-, replicate 3 CKO male mice, DAPI+ Ne…
- GSM8435160 CKO female mice, DAPI+, replicate 1 CKO female mice, DAPI+,
- GSM8435161 CKO female mice, DAPI+, replicate 2 CKO female mice, DAPI+,
- GSM8435162 CKO female mice, DAPI+, replicate 3 CKO female mice, DAPI+,
- GSM8435163 CKO female mice, DAPI+ NeuN-, replicate 1 CKO female mice, DAPI+ …
- GSM8435164 CKO female mice, DAPI+ NeuN-, replicate 2 CKO female mice, DAPI+ …
- GSM8435165 CKO female mice, DAPI+ NeuN-, replicate 3 CKO female mice, DAPI+ …
- GSM8435166 ADCon male mice, DAPI+, replicate 1 ADCon male mice, DAPI+,
- GSM8435167 Con male mice, DAPI+, replicate 4 Con male mice, DAPI+,
- GSM8435168 ADCon male mice, DAPI+, replicate 3 ADCon male mice, DAPI+,
- GSM8435169 ADCon male mice, DAPI+ NeuN-, replicate 1 ADCon male mice, DAPI+ …
- GSM8435170 Con male mice, DAPI+ NeuN-, replicate 4 Con male mice, DAPI+ Ne…
- GSM8435171 ADCon male mice, DAPI+ NeuN-, replicate 3 ADCon male mice, DAPI+ …
- GSM8435172 ADCon female mice, DAPI+, replicate 1 ADCon female mice, DAPI…
- GSM8435173 ADCon female mice, DAPI+, replicate 2 ADCon female mice, DAPI…
- GSM8435174 ADCon female mice, DAPI+, replicate 3 ADCon female mice, DAPI…
- GSM8435175 ADCon female mice, DAPI+ NeuN-, replicate 1 ADCon female mice, DAPI…
- GSM8435176 ADCon female mice, DAPI+ NeuN-, replicate 2 ADCon female mice, DAPI…
- GSM8435177 ADCon female mice, DAPI+ NeuN-, replicate 3 ADCon female mice, DAPI…
- GSM8435178 ADCKO male mice, DAPI+, replicate 1 ADCKO male mice, DAPI+,
- GSM8435179 ADCKO male mice, DAPI+, replicate 2 ADCKO male mice, DAPI+,
- GSM8435180 ADCKO male mice, DAPI+, replicate 3 ADCKO male mice, DAPI+,
- GSM8435181 ADCKO male mice, DAPI+ NeuN-, replicate 1 ADCKO male mice, DAPI+ …
+ 8 more — browse all 48 samples with per-sample file links →
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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.