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Single cell ATAC sequencing in Skeletal cells

GSE274263 Mus musculus Genome binding/occupancy profiling by high throughput sequencing; Other 9 samples Submitted 2025/02/01 Platform GPL24247
Summary
To determine lineage and differentiation relationship among skeletal cells in native environment, we performed tracking of clonal and sub-clonal mitochondrial mutations across population. Specifically, we utilized PolGD257A/D257A mice with a mitochondrial DNA “mutator” phenotype that provide a rich substrate of somatic mutations to facilitate clonal lineage tracing among skeletal cells. Here, CD24+CD29+SSCs, CD29+COPs, CD24+CAPs, CD24-CD29-cells, THY+ osteolineage cells, CD200-CD105+ adipolineage cells and CD45+ hematopoietic cells were isolated from the femurs of 3-month-old PolGD257A/D257A mice, co-labelled with both FACS and CITE-seq antibodies, and were subjected to a modified single cell ATAC-seq method for single cell sequencing of mtDNA variants. Our data establish that CD24+CD29+SSCs share a clonal lineage with adipolineage and osteolineage cells, providing the first evidence of clonal multipotency in skeletal cells and thus, establishes the direct in vivo evidence for our model of skeletal cell differentiation.
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Direct links to NCBI, no account and no request form: the whole study as GSE274263_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 9 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1145471 and SRA study SRP524816. Searching any of these in the dataset finder brings you back here.

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