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B-cell Interleukin 1 Receptor 1 modulates the female adipose tissue immune microenvironment during aging

GSE274935 Mus musculus Expression profiling by high throughput sequencing 8 samples Submitted 2024/11/19 Platform GPL24247
Summary
The Nod like receptor protein 3 (NLRP3) inflammasome and its product interleukin-1β (IL-1β) drive inflammaging in visceral adipose tissue (vWAT) and contribute to the expansion of interleukin-1 receptor 1 (Il1r1) positive aged adipose B-cells (AABs). AABs promote metabolic dysfunction and inflammation under inflammatory challenges. However, it is unclear whether IL-1β contributes to AAB-associated inflammation during aging. Using a B-cell specific knockout of Il1r1 (BKO mice), we characterized old vWAT in the absence of IL-1β – B-cell signaling. In addition to sex-specific metabolic improvements in females, we identified a reduction in the proportion of B-cells and a sex-specific increase in the B1:B2 B-cell ratio in BKO vWAT. Using single cell RNA-sequencing of vWAT immune cells, we observed that BKO differentially affected inflammatory signaling in vWAT immune cells. These data suggest that IL-1β – B-cell signaling supports the inflammatory response in multiple cell types and provides insight into the complex microenvironment in aged vWAT.
Published in
B-cell interleukin 1 receptor 1 modulates the female adipose tissue immune microenvironment during aging
Carey A, Pitcher LE, Jang IH et al. · Journal of leukocyte biology 2025 · PMID 39378334 · doi:10.1093/jleuko/qiae219
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Also filed as BioProject PRJNA1148505 and SRA study SRP526655. Searching any of these in the dataset finder brings you back here.

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