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Comparative bulk RNAseq between EpH4 WT and Cldn-null cells

GSE274940 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2025/09/24 Platform GPL34290
Summary
The claudin (CLDN) family, comprising 27 members, includes crucial cell–cell adhesion molecules that form tight junctions (TJs), essential for paracellular barrier and channel functions. The functional diversity among CLDNs may lead to diversity of TJs, contributing to various biological systems and pathological processes. To characterize the functional diversity of CLDNs, we developed a first-ever complete Cldn-null EpH4 epithelial cell line. This cell line was used for the expression of single Cldn family members, uncovering their specific intrinsic properties. Unlike the conventional binary classification of CLDNs as either paracellular barrier or channel forming, we discovered a novel classification axis where CLDNs form TJs autonomously or non-autonomously, exhibiting extensive variation in ion conductivity and selectivity, leading to an innovative classification. This classification offers a fundamental framework for understanding the mechanisms underlying TJ diversity in vivo, and provides a basis for the development of therapies targeting the epithelial barrier.
Published in
Functional landscape of mechanistic diversity in 27 claudin family members at tight junctions
Kashihara H, Tanaka H, Kitamata M et al. · Science advances 2025 · PMID 41171911 · doi:10.1126/sciadv.adx7431
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Direct links to NCBI, no account and no request form: the whole study as GSE274940_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1148533 and SRA study SRP526656. Searching any of these in the dataset finder brings you back here.

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