Transcriptome analysis reveals the molecular mechanism of Baduanjin in improving type 2 diabetes

Among the many clinical practices that use Baduanjin as a treatment for type 2 diabetes patients, it has shown to be effective, but there is still a lack of research on molecular regulation mechanisms. We detected the levels of lncRNA, circRNA, and mRNA in the blood of type 2 diabetes patients before and after the intervention of Baduanjin for 12 weeks. Our analysis used sequencing and bioinformatics methods to identify differentially expressed genes, circRNAs, and lncRNAs possibly related to the regulation of diabetes by Baduanjin. From peripheral blood of patients with diabetes before and after Baduanjin intervention, 4244 DE mRNAs, 463 lncRNAs, and 516 circRNAs were determined. Systematic bioinformatics analysis revealed that the DE genes are functionally rich in oxidative phosphorylation and cytokine-cytokine receptor interaction. In addition, the results indicated that the potential ENST00000512668-G0S2 interplay, which has been methodically validated in the lncRNA prediction database, participates in Baduanjin regulation of diabetes by altering oxidative phosphorylation in patients with diabetes. This finding provides molecular targets for improving the use of Baduanjin as adjunctive therapy for diabetes.