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Tachykinin signaling defines distinct populations of glia in the enteric nervous system [scRNA-seq]

GSE275795 Mus musculus Expression profiling by high throughput sequencing 3 samples Submitted 2026/01/28 Platform GPL19057
Summary
One of the largest populations of glia outside the brain is in the gut. These enteric glia are involved in many functions from intestinal peristalsis to immunity, yet it is unclear if there are defined types with distinct roles in homeostasis. Comparing glia from divergent microenvironments in the mouse intestine, we found that mucosal glia resembled microglia and macrophages, while muscularis glia resembled satellite glia. Tacr3, encoding the receptor for neuropeptide Neurokinin B (NKB), was enriched within muscularis glia associated with neuronal soma and was undetectable in extraintestinal glia. Genetic or pharmacological manipulation of NKB-TACR3 signaling disrupted establishment of enteric glial populations during postnatal development, and dynamically modulated intestinal motor behaviors in adult mice. Collectively, we delineate spatially, transcriptionally and functionally distinct populations of enteric glia, identify one as an unanticipated target of TACR3 antagonists in clinical use, and establish this pathway as necessary for enteric glial diversification and function.
Published in
Tachykinin signaling defines distinct populations of glia in the enteric nervous system
Muppirala AN, Mitchell PE, Courtney E et al. · Neuron 2026 · PMID 41512855 · doi:10.1016/j.neuron.2025.11.030
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Also filed as BioProject PRJNA1153002 and SRA study SRP528972. Searching any of these in the dataset finder brings you back here.

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