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Mitochondrial metabolism regulates the immunogenic responsiveness of dendritic cells [RNA-seq]

GSE276024 Mus musculus (mouse) RNA-seq (gene expression) 24 samples Submitted 2026/04/14 Platform GPL21103
At a glance

RNA-seq (gene expression) · mouse · 24 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
24 samples: 5 conditions, mostly in triplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Mitochondrial metabolism regulates the immunogenic responsiveness of dendritic cellsCell metabolism · 2026
Summary (written by the submitters)
Activation of dendritic cells (DCs) is linked to increased glycolysis. However, the role of the mitochondrial electron transport chain (ETC) in conventional DC subset function is poorly defined. Here, we revealed that immunogenic activation elevates late oxygen consumption in cDC1s, but not cDC2s. To investigate the relevance of the ETC in DC immunogenicity, we generated mice with impaired ETC complex III function in DCs.
Read the full summary ↓
ETC complex III impairment attenuated poised or adjuvant-triggered cDC1 activation, migration and capability to prime T cells for anti-cancer immunity, while it had a mild effect on cDC2s. Mechanistically, loss of complex III in cDC1s led to a deregulated redox and metabolite balance causing DNA hypermethylation of PU.1-binding regions. This DNA hypermethylation diminished early stimulus-induced gene expression linked to immunogenic responsiveness of cDC1s, which was rescued via ectopic expression of alternative oxidase. Our findings show how the ETC differentially regulates the immunogenic function of cDC subsets.
Published in
Mitochondrial metabolism regulates the immunogenic responsiveness of dendritic cells
Heras-Murillo I, Mañanes D, Calafell-Segura J et al. · Cell metabolism 2026 · PMID 41990746 · doi:10.1016/j.cmet.2026.03.012
This dataset
Open GSE276024 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE276024_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1154295 and SRA study SRP529481. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41990746) ↗
Study design
5 conditions, mostly in triplicate
C+R848, 1.5h, ×12 CD8+ cDC1, Uqcrqf/f, PBS, ×3 CD8+ cDC1, Itgax-Uqcrqf/f, PBS, ×3 CD11b+ cDC2, Uqcrqf/f, PBS, ×3 CD11b+ cDC2, Itgax-Uqcrqf/f, PBS, ×2 CD11b+ cDC2,Itgax-Uqcrqf/f, PBS, ×1

Supports a between-group comparison across 23 samples.

How we read this: 5 replicated groups read from 24 sample titles; they account for 23 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

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