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Beta-Adrenergic Stimulation and MYH7 G256E Mutant Gene Dosage Drive Hypertrophic Cardiomyopathy Phenotype Penetrance

GSE276210 Homo sapiens (human) Single-cell RNA-seq 14 samples Submitted 2025/08/01 Platform GPL34284Platform GPL24676
At a glance

Single-cell RNA-seq · human · 14 samples

What it is
Single-cell RNA-seqGene activity measured in thousands of individual cells, so cell types can be told apart. Read from the summary: GEO's own type field only says 'expression profiling by sequencing', so confirm it in the paper.
Organism
Homo sapiens (human)
Samples
14 samples: 2 × hiPSC-CM, MYH7 G256E HET & WT Iso treated, Day 30, Gene-Expression, vs 2 × hiPSC-CM, MYH7 G256E HET & WT Iso treated, Day 30, FB-Expression, vs 2 × hiPSC-CM, MYH7 H251N HET & WT, Day 30, Gene-Expression, vs 2 × hiPSC-CM, MYH7 H251N HET & WT, Day 30, FB-Expression,Groups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Summary (written by the submitters)
We characterized the baseline transcriptomic phenotype of day30 iPSC-Cardiomyocytes carrying hypertrophic cardiomyopathy associated heterozygous mutations MYH7 G256E and H251N using scRNAseq. We additionally analzyed trancriptomic signatures of HOM MYH7 G256E iPSC-CMs, and of HET MYH7 G256E iPSC-CMs after isopreterenol treatment.
This dataset
Open GSE276210 in finder → Find more human RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE276210_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: Single-cell studies usually ship their counts as 10x files (matrix.mtx, barcodes.tsv, features.tsv), an .h5 file, or an .rds / .h5ad object that Seurat or Scanpy can open. Look for those among the supplementary files.

Also filed as BioProject PRJNA1155708 and SRA study SRP530101. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 42282692) ↗
Study design
2 × hiPSC-CM, MYH7 G256E HET & WT Iso treated, Day 30, Gene-Expression, vs 2 × hiPSC-CM, MYH7 G256E HET & WT Iso treated, Day 30, FB-Expression, vs 2 × hiPSC-CM, MYH7 H251N HET & WT, Day 30, Gene-Expression, vs 2 × hiPSC-CM, MYH7 H251N HET & WT, Day 30, FB-Expression,

Supports a between-group comparison across 8 samples.

How we read this: 4 replicated groups read from 14 sample titles; they account for 8 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

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