Transcriptional profiling of isolated T cells of healthy individuals after treatment with supernatant of peripheral blood mononuclear cells (PBMCs) of the same individuals treated overnight with soluble CD14 (sCD14)
RNA-seq (gene expression) · human · 12 samples
- What it is
- RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
- Organism
- Homo sapiens (human)
- Samples
- 12 samples: 12 samples, each with a distinct title — no replicated groupsGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- Soluble CD14 promotes Th17 expansion and differentiation through gamma-aminobutyric acid and expands infidel innate lymphoid cellsPNAS nexus · 2026
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE277708_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.
Also filed as BioProject PRJNA1163401 and SRA study SRP533917. Searching any of these in the dataset finder brings you back here.
Any grouping that defines a comparison lives in the sample characteristics, which GEO records separately from the titles.
How we read this: Read from 12 sample titles: 12 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM8528143 1_Stim
- GSM8528144 1_Stim+Sup
- GSM8528145 2_Stim
- GSM8528146 2_Stim+Sup
- GSM8528147 3_Stim
- GSM8528148 3_Stim+Sup
- GSM8528149 4_Stim
- GSM8528150 4_Stim+Sup
- GSM8528151 5_Stim
- GSM8528152 5_Stim+Sup
- GSM8528153 6_Stim
- GSM8528154 6_Stim+Sup
- GSE277711 Transcriptional profiling of T cells of healthy individuals after treatment with supernatant of PBMCs of the same individuals treated overnight with sCD14 with isolated T cells cultured in TH17 polarizing condition and T cells treated with GABA overnight 28 samples shares scd14, cd14, individuals, supernatant, treatment, overnight
- GSE279719 Human CD14+ monocytes from early inflammatory arthritis patients before and after a 5day-and 30day-period of methotrexate treatment 72 samples shares cd14, treatment
- GSE217459 Gene expression of CD14+ peripheral blood monocytes in different phases of chronic HBV infection 7 samples shares cd14, peripheral
- GSE253495 Human CD14+ monocytes from rheumatoid arthritis patients before and after a 3-month period of Upadacitinib treatment 6 samples shares cd14, treatment
- GSE230276 Distinct blood CD3+ CD14+ T Cell-Monocyte complexes harbor HIV and are dynamic, glucose-dependent and increased in individuals with glucose intolerance 3 samples shares cd14, individuals
- GSE39643 NanoString miRNA profiling of peripheral blood sorted CD14+CD16- monocytes from amyotrophic lateral sclerosis, multiple sclerosis and healthy control subjects 24 samples shares cd14, peripheral
- GSE7893 Differential gene expression in peripheral blood CD14+ leukocytes from SRESS study particpants 21 samples shares cd14, peripheral
- GSE234521 DOGMA-seq of opioid addiction treatment in HIV-1 infected individuals 27 samples shares individuals, treatment
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.