Cryptic splice sites generate heterogeneous EZH2 isoforms with distinct functions in embryonic development [RNA-seq]
RNA-seq (gene expression) · mouse · 12 samples
- What it is
- RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
- Organism
- Mus musculus (mouse)
- Samples
- 12 samples: 3 × Ezh2a, mutation, vs 3 × Ezh2a, wild type, vs 3 × Ezh2b, mutation, vs 3 × Ezh2b, wild type,Groups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- EZH2 variants derived from cryptic splice sites govern distinct epigenetic patterns during embryonic developmentNucleic acids research · 2025
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE278314_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.
Also filed as BioProject PRJNA1166315 and SRA study SRP535361. Searching any of these in the dataset finder brings you back here.
Supports a between-group comparison across 12 samples.
How we read this: 4 replicated groups read from 12 sample titles; they account for 12 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM8544681 Ezh2a, mutation, rep1 Ezh2a, mutation,
- GSM8544682 Ezh2a, mutation, rep2 Ezh2a, mutation,
- GSM8544683 Ezh2a, mutation, rep3 Ezh2a, mutation,
- GSM8544684 Ezh2a, wild type, rep1 Ezh2a, wild type,
- GSM8544685 Ezh2a, wild type, rep2 Ezh2a, wild type,
- GSM8544686 Ezh2a, wild type, rep3 Ezh2a, wild type,
- GSM8544687 Ezh2b, mutation, rep1 Ezh2b, mutation,
- GSM8544688 Ezh2b, mutation, rep2 Ezh2b, mutation,
- GSM8544689 Ezh2b, mutation, rep3 Ezh2b, mutation,
- GSM8544690 Ezh2b, wild type, rep1 Ezh2b, wild type,
- GSM8544691 Ezh2b, wild type, rep2 Ezh2b, wild type,
- GSM8544692 Ezh2b, wild type, rep3 Ezh2b, wild type,
- GSE302605 Functional Comparison to Ezh2 Reveals PRC2-Independent Functions of Jarid2 in Hematopoietic Stem Cell Lineage Commitment 23 samples shares ezh2, functions
- GSE270036 Investigate the functions of core pluripotency transcription factors in embryonic stem cells 30 samples shares functions, embryonic
- GSE285025 TEAD switches interacting partners along neural progenitor lineage progression to execute distinct functions 23 samples shares functions, distinct
- GSE271800 Diploid embryo complementation of embryonic stem cells efficiently generates a homozygous Gjb2 mutant deaf mouse model 131 samples shares embryonic, generate
- GSE310209 Opposing functions of AEBP2 isoforms fine-tune PRC2 catalytic activity 72 samples shares functions, isoforms
- GSE297143 EZH2 alters aggressive variant prostate cancer subtype evolution, but is not required for neuroendocrine prostate cancer [RNAseq] 54 samples shares ezh2
- GSE264171 Effects at the transcriptomic level after knockout of Eed, Ezh2, Brd8 or Nr6a1 in spinal cord neural progenitor differentiation [bulkRNAseq] 41 samples shares ezh2
- GSE304090 Pharmacological inhibition of the epigenetic enzyme EZH2 attenuates cardiac dysfunction and mitigates lipid metabolism perturbations in a murine model of myocardial infarction 15 samples shares ezh2
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.