An unbiased genomewide screen uncovers 7 genes that drive hematopoietic stem cell fate from mouse embryonic stem cells
RNA-seq (gene expression) · mouse · 16 samples
- What it is
- RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
- Organism
- Mus musculus (mouse)
- Samples
- 16 samples: 4 × CD45 BFP Bone marrow 2ary Transplant 12 weeks vs 3 × EBs 144h WT vs 3 × EBs 144h SADEiGEN vs 2 × KDR 144h EbsGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- An unbiased genomewide screen uncovers 7 genes that drive hematopoietic stem cell fate from mouse embryonic stem cellsBlood · 2025
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE278947_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.
Also filed as BioProject PRJNA1169803 and SRA study SRP525386. Searching any of these in the dataset finder brings you back here.
Supports a between-group comparison across 12 samples.
How we read this: 4 replicated groups read from 16 sample titles; they account for 12 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM8449002 CD45 BFP Bone marrow 2ary Transplant 12 weeks rep1 CD45 BFP Bone marrow 2a…
- GSM8449003 CD45 BFP Bone marrow 2ary Transplant 12 weeks rep2 CD45 BFP Bone marrow 2a…
- GSM8449004 CD45 BFP Bone marrow 2ary Transplant 12 weeks rep3 CD45 BFP Bone marrow 2a…
- GSM8449005 CD45 BFP Bone marrow 2ary Transplant 12 weeks rep4 CD45 BFP Bone marrow 2a…
- GSM8449006 CD45 BFP Bone marrow 2ary Transplant 16 weeks rep1
- GSM8449007 CD45 BFP Bone marrow 1ary Transplant 4 weeks rep1
- GSM8449008 KDR 144h Ebs rep1 KDR 144h Ebs
- GSM8449009 KDR 144h Ebs rep2 KDR 144h Ebs
- GSM8449010 BM 4.5mo PostTransp CD45 BFP-
- GSM8449011 BM 4.5mo PostTransp CD45 BFP+
- GSM8449012 EBs 144h WT rep1 EBs 144h WT
- GSM8449013 EBs 144h WT rep2 EBs 144h WT
- GSM8449014 EBs 144h WT rep3 EBs 144h WT
- GSM8449015 EBs 144h SADEiGEN rep1 EBs 144h SADEiGEN
- GSM8449016 EBs 144h SADEiGEN rep2 EBs 144h SADEiGEN
- GSM8449017 EBs 144h SADEiGEN rep3 EBs 144h SADEiGEN
- GSE266509 A Bcl11a-driven mechanism maintaining hematopoietic stem cell functionality gives rise to dysfunction during aging [RNA-Seq] 52 samples shares hematopoietic, drive, stem
- GSE277681 Premature senescence impairs hematopoietic stem cell function during sickle cell disease in mice and humans (Mouse CITE-Seq) 32 samples shares hematopoietic, stem
- GSE229137 Autophagy engaging and non-engaging hematopoietic stem cells 607 samples shares hematopoietic, stem
- GSE265909 yHSC and oHSC scRNAseq with oHSC AThi ATlo Index Sorting. Assessing the transcriptional changes associated with autophagy engagement in old hematopoietic stem cells at single cell resolution. [scRNA-Seq] 563 samples shares hematopoietic, stem
- GSE202079 Short term perturbation of mouse embryonic stem cells with morphogens and small molecule compounds 208 samples shares embryonic, stem
- GSE272452 Generation of germinal-vesicle oocytes from mouse embryonic stem cells under an ovarian soma-free condition 202 samples shares embryonic, stem
- GSE285231 Systems genetics reveals the influence of eQTLs in mouse embryonic stem cells on transcriptional variation later in differentiated neural progenitor cells 186 samples shares embryonic, stem
- GSE271800 Diploid embryo complementation of embryonic stem cells efficiently generates a homozygous Gjb2 mutant deaf mouse model 131 samples shares embryonic, stem
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.