Temozolomide Induces Glioblastoma Dedifferentiation into Highly Malignant Stem-like Cells Through Senescence-Associated Reprogramming via HIF1α/HIF2α Regulation [RNA-seq]
RNA-seq (gene expression) · human · 12 samples
- What it is
- RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
- Organism
- Homo sapiens (human)
- Samples
- 12 samples: 3 × U87_sensitive_RNA-seq vs 3 × U87_resistant_RNA-seq vs 3 × U118_sensitive_RNA-seq vs 3 × U118_resistant_RNA-seqGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- Temozolomide promotes glioblastoma stemness expression through senescence-associated reprogramming via HIF1α/HIF2α regulationCell death & disease · 2025
Read the full summary ↓
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE282387_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.
Also filed as BioProject PRJNA1188485 and SRA study SRP546377. Searching any of these in the dataset finder brings you back here.
Supports a between-group comparison across 12 samples.
How we read this: 4 replicated groups read from 12 sample titles; they account for 12 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM8642349 U87_sensitive_RNA-seq_1 U87_sensitive_RNA-seq
- GSM8642350 U87_sensitive_RNA-seq_2 U87_sensitive_RNA-seq
- GSM8642351 U87_sensitive_RNA-seq_3 U87_sensitive_RNA-seq
- GSM8642352 U87_resistant_RNA-seq_1 U87_resistant_RNA-seq
- GSM8642353 U87_resistant_RNA-seq_2 U87_resistant_RNA-seq
- GSM8642354 U87_resistant_RNA-seq_3 U87_resistant_RNA-seq
- GSM8642355 U118_sensitive_RNA-seq_1 U118_sensitive_RNA-seq
- GSM8642356 U118_sensitive_RNA-seq_2 U118_sensitive_RNA-seq
- GSM8642357 U118_sensitive_RNA-seq_3 U118_sensitive_RNA-seq
- GSM8642358 U118_resistant_RNA-seq_1 U118_resistant_RNA-seq
- GSM8642359 U118_resistant_RNA-seq_2 U118_resistant_RNA-seq
- GSM8642360 U118_resistant_RNA-seq_3 U118_resistant_RNA-seq
- GSE295278 Hyperbaric Oxygen Promotes Both the Proliferation and Chemosensitization of Glioblastoma Cells by Inhibiting HIF1α/HIF2α-ABCG2 12 samples shares hif1, hif2, glioblastoma
- GSE328454 Gene expression profiling of A498 clear cell renal cell carcinoma cells following HIF1A and HIF2A knockdown 12 samples shares hif1, hif2
- GSE292666 MYB and HIF1α crosstalk drives hypoxia-induced transcriptional reprogramming and adaptive signaling alterations in pancreatic cancer [RNA-seq] 12 samples shares hif1, reprogramming
- GSE334976 HEXB promotes tumor progression of glioblastoma through the HIF1α-CA9 pathway as a therapeutic approach 9 samples shares hif1, glioblastoma
- GSE255501 LncRNA LUCAT1 Promotes Glioblastoma Progression by Enhancing HIF1⍺ Activity [RNA-seq] 8 samples shares hif1, glioblastoma
- GSE292665 MYB and HIF1α crosstalk drives hypoxia-induced transcriptional reprogramming and adaptive signaling alterations in pancreatic cancer [ChIP-seq] 27 samples shares hif1, reprogramming
- GSE255502 LncRNA LUCAT1 Promotes Glioblastoma Progression by Enhancing HIF1⍺ Activity [ChIP-seq] 8 samples shares hif1, glioblastoma
- GSE327285 Reprogramming Dedifferentiation Regulatory Networks Preserves Human Chondrocyte Phenotypes (RNA-Seq) 12 samples shares dedifferentiation, reprogramming
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.