Age and cell type specific specific effects of histone variant H2BE in the brain [Intact_RNAseq]
Single-nucleus RNA-seq · mouse · 12 samples
- What it is
- Single-nucleus RNA-seqGene activity measured in individual nuclei (used when whole cells are hard to isolate, for example from frozen tissue). Read from the summary: GEO's own type field only says 'expression profiling by sequencing', so confirm it in the paper.
- Organism
- Mus musculus (mouse)
- Samples
- 12 samples: 4 × Male P0 vs 4 × Male P14 vs 4 × Male P28Groups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- Age- and cell-type-specific effects of histone variant H2BE in the brainCell reports · 2025
Read the full summary ↓
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE282467_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: Single-cell studies usually ship their counts as 10x files (matrix.mtx, barcodes.tsv, features.tsv), an .h5 file, or an .rds / .h5ad object that Seurat or Scanpy can open. Look for those among the supplementary files.
Also filed as BioProject PRJNA1188837 and SRA study SRP546649. Searching any of these in the dataset finder brings you back here.
Supports a between-group comparison across 12 samples.
How we read this: 3 replicated groups read from 12 sample titles; they account for 12 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM8644115 Male P0 bio rep 1 Male P0
- GSM8644116 Male P0 bio rep 2 Male P0
- GSM8644117 Male P0 bio rep 3 Male P0
- GSM8644118 Male P0 bio rep 4 Male P0
- GSM8644119 Male P14 bio rep 1 Male P14
- GSM8644120 Male P14 bio rep 2 Male P14
- GSM8644121 Male P14 bio rep 3 Male P14
- GSM8644122 Male P14 bio rep 4 Male P14
- GSM8644123 Male P28 bio rep 1 Male P28
- GSM8644124 Male P28 bio rep 2 Male P28
- GSM8644125 Male P28 bio rep 3 Male P28
- GSM8644126 Male P28 bio rep 4 Male P28
- GSE267938 Functional optic tract rewiring via subtype- and target-specific axonal regeneration and presynaptic activity enhancement 256 samples shares specific, type
- GSE297143 EZH2 alters aggressive variant prostate cancer subtype evolution, but is not required for neuroendocrine prostate cancer [RNAseq] 54 samples shares variant, type
- GSE242179 Role of the histone variant H2A.Z.1 in memory, transcription, and alternative splicing is mediated by lysine modification 40 samples shares histone, variant
- GSE269748 A single-cell atlas deconstructs heterogeneity across multiple models in murine traumatic brain injury and identifies novel cell-specific targets 36 samples shares specific, brain
- GSE306031 Single-Nucleus Transcriptomics Reveals Cell Type-Specific Remodeling and Epilepsy Associated Microglia 31 samples shares specific, type
- GSE241176 Fibroblast-specific TGF-b signaling mediates dysfunction, fibrosis and hypertrophy in a mouse model of obesity-associated type 2 diabetes [RNA-seq: Smad3 in dbdb] 12 samples shares specific, type
- GSE331488 A global view of cell-type-specific temporal dynamics in mammalian aging [I] 771 samples shares specific, type
- GSE331489 A global view of cell-type-specific temporal dynamics in mammalian aging [II] 725 samples shares specific, type
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.