RNA-seq analysis of ex vivo microtumor models comprising control or Arpc4 knockout (KO) PDAC cells

The loss of Arpc4 resulted in reduced expression of other Arp2/3 complex components, rendering the Arp2/3 complex nonfunctional. The ex vivo microtumor(3D) models were composed of either control or Arpc4 knockout (KO) KP 8025 cells, mouse primary pancreatic stellate cells (PSCs), and bone marrow-derived macrophages. To identify genes affected by Arpc4 knockout in this model, RNA-seq analysis was performed on bulk samples. R254 cell line which was derived from primary cells isolated from genetically engineered p48Cre/+; LSL-KrasG12D/+; p53flox/flox (KPC) mice, and 8025 cell line which was derived from primary cells isolated from genetically engineered p48Cre/+; LSL-KrasG12D/+ (KC) mice were also conducted with RNA seq.