Single-cell RNA sequencing analysis of baicalein’s immunomodulatory effects in lung graft ischemia-reperfusion injury

This dataset encompasses single-cell RNA sequencing data from lung graft samples subjected to ischemia-reperfusion injury (IRI) with and without baicalein treatment, as well as sham-operated controls. A total of 105,337 cells were initially obtained across nine samples, followed by rigorous quality control measures that yielded 83,590 high-quality cells for downstream analysis. The analysis identified 14 classical immune cell types and 36 subpopulations, highlighting significant differences in neutrophil composition and gene expression between baicalein-treated (BI) and untreated IRI (IR) groups. Specifically, the BI group exhibited a marked reduction in neutrophil abundance and a lower activation level of the AGE-RAGE signaling pathway. Differentially expressed gene analysis in neutrophils identified 263 upregulated and 337 downregulated genes in the BI group compared to the IR group, with functional enrichment analysis revealing pathways associated with immune regulation and cellular stress responses. These findings provide valuable insights into the immunomodulatory effects of baicalein during lung graft IRI and offer a resource for further investigation into therapeutic mechanisms.