Transcriptome of Ag-specific B cells upon stimulation with soluble or particulate Ag

Primary B cells from MD4 mice have transgenic B cell receptors that recognize hen egg lysozyme (HEL). Previous studies have shown that antigens presented in different formats induce qualitatively different signaling responses in B cells. Here, we compare the transcriptomes induced by the following stimuli: LPS (10 mg/mL), particulate HEL (1pM liposomes with reduced affinity HEL-3X protein conjugated to surface at epitope density 243), soluble HEL (1mg/mL), and co-stimulation with soluble HEL and anti-CD40 (100 ng/mL).