GEO series

Orai1 in pancreatic ductal cells mediates pancreas-intestinal crosstalk in experimental acute pancreatitis via the secretion of antimicrobial peptide REG3B in pancreatic juice

GSE285771 Mus musculus (mouse) RNA-seq (gene expression) 18 samples Submitted 2026/01/07 Platform GPL24247

At a glance

RNA-seq (gene expression) · mouse · 18 samples

What it is: RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism: Mus musculus (mouse)
Samples: 18 samples: 5 × Orai1f/f mouse, control vs 5 × Orai1Sox9 mouse, control vs 5 × Orai1f/f mouse, AP vs 3 × Orai1Sox9 mouse, APGroups are read from the sample names, not from the paper. Check the list below.
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.

Summary (written by the submitters)

Pancreatic ductal epithelial cells (PDECs) play a crucial role in the development of acute pancreatitis (AP). We and others have previously shown that Orai1 inhibitor confers protection against AP severity. In this study, we aim to investigate the effect and the underlying mechanisms of Orai1 in PDECs on experimental AP and its-associated intestinal damage.

Read the full summary ↓

We found that Orai1 deletion in PDECs markedly mitigated the prolonged elevation of calcium and preserved the decreased mitochondrial membrane potential due to sodium taurocholate (NaT) or palmitoleic acid (POA) exposure. PDECs-specific Orai1 deletion notably lessened pancreatitis severity and restored the impaired pancreatic juice secretion during AP. Increased expression of tight junction proteins and decreased intestinal permeability and inflammation were observed in duodenal and colonic tissue of PDECs-specific Orai1 knockout mice. Furthermore, the deletion of Orai1 protected against pancreatitis-associated dysbiosis in the duodenum, but not in the colon. Mechanically, this protective effect was partially attributed to the enhanced secretion of REG3B in pancreatic juice during AP. Finally, intraperitoneal administration of REG3B partially alleviated AP severity and pancreatitis-associated intestinal injury and inflammation. Collectively, these findings suggest that Orai1 inhibitor may serve as potential therapeutic strategy for the management of AP and associated intestinal injury.

This dataset

Open GSE285771 in finder → Find more mouse RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE285771_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1205402. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗

Study design

5 × Orai1f/f mouse, control vs 5 × Orai1Sox9 mouse, control vs 5 × Orai1f/f mouse, AP vs 3 × Orai1Sox9 mouse, AP

Supports a between-group comparison across 18 samples.

How we read this: 4 replicated groups read from 18 sample titles; they account for 18 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

GSM8708777 Orai1f/f mouse, control1 Orai1f/f mouse, control
GSM8708778 Orai1f/f mouse, control2 Orai1f/f mouse, control
GSM8708779 Orai1f/f mouse, control3 Orai1f/f mouse, control
GSM8708780 Orai1f/f mouse, control4 Orai1f/f mouse, control
GSM8708781 Orai1f/f mouse, control5 Orai1f/f mouse, control
GSM8708782 Orai1Sox9 mouse, control1 Orai1Sox9 mouse, control
GSM8708783 Orai1Sox9 mouse, control2 Orai1Sox9 mouse, control
GSM8708784 Orai1Sox9 mouse, control3 Orai1Sox9 mouse, control
GSM8708785 Orai1Sox9 mouse, control4 Orai1Sox9 mouse, control
GSM8708786 Orai1Sox9 mouse, control5 Orai1Sox9 mouse, control
GSM8708787 Orai1f/f mouse, AP1 Orai1f/f mouse, AP
GSM8708788 Orai1f/f mouse, AP2 Orai1f/f mouse, AP
GSM8708789 Orai1f/f mouse, AP3 Orai1f/f mouse, AP
GSM8708790 Orai1f/f mouse, AP4 Orai1f/f mouse, AP
GSM8708791 Orai1f/f mouse, AP5 Orai1f/f mouse, AP
GSM8708792 Orai1Sox9 mouse, AP1 Orai1Sox9 mouse, AP
GSM8708793 Orai1Sox9 mouse, AP2 Orai1Sox9 mouse, AP
GSM8708794 Orai1Sox9 mouse, AP3 Orai1Sox9 mouse, AP

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