GEO series

Role of Hepatocyte RIPK1 in Maintaining Liver Homeostasis during Metabolic Challenges [scRNA-seq]

GSE286072 Mus musculus (mouse) Single-cell RNA-seq 2 samples Submitted 2025/01/11 Platform GPL24247

At a glance

Single-cell RNA-seq · mouse · 2 samples

What it is: Single-cell RNA-seqGene activity measured in thousands of individual cells, so cell types can be told apart.
Organism: Mus musculus (mouse)
Samples: 2 samples
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper: Role of hepatocyte RIPK1 in maintaining liver homeostasis during metabolic challengeseLife · 2025

Summary (written by the submitters)

In this study, we identified Receptor interacting protein kinase 1 (RIPK1) in hepatocytes as a critical regulator in preserving hepatic homeostasis during metabolic challenges, such as short-term fasting or high-fat dieting. Our results demonstrated that hepatocyte-specific deficiency of RIPK1 sensitized the liver to short-term fasting-induced liver injury and hepatocyte apoptosis in both male and female mice.

Read the full summary ↓

Despite being a common physiological stressor that typically does not induce liver inflammation, short-term fasting triggered hepatic inflammation and compensatory proliferation in hepatocyte-specific RIPK1-deficient (Ripk1Δhep) mice. Transcriptomic analysis revealed that short-term fasting oriented the hepatic microenvironment into an inflammatory state in Ripk1Δhep mice, with upregulated expression of inflammation and immune cell recruitment-associated genes. Single-cell RNA sequencing further confirmed the altered cellular composition in the liver of Ripk1Δhep mice during fasting, highlighting the increased recruitment of macrophages to the liver.

Published in

Role of hepatocyte RIPK1 in maintaining liver homeostasis during metabolic challenges

Zhang W, Liu H, Zhang D et al. · eLife 2025 · PMID 39886919 · doi:10.7554/eLife.96798

This dataset

Open GSE286072 in finder → Find more mouse RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE286072_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: Single-cell studies usually ship their counts as 10x files (matrix.mtx, barcodes.tsv, features.tsv), an .h5 file, or an .rds / .h5ad object that Seurat or Scanpy can open. Look for those among the supplementary files.

Also filed as BioProject PRJNA1207579 and SRA study SRP555874. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 39886919) ↗

Samples in this study

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+ 2 more — browse all 2 samples with per-sample file links →

Datasets on a similar topic

GSE286073 Role of Hepatocyte RIPK1 in Maintaining Liver Homeostasis during Metabolic Challenges [RNA-seq] 10 samples shares ripk1, maintaining, homeostasis, hepatocyte, challenges, liver
GSE279700 Cooperation of TRADD- and RIPK1-dependent Cell Death Pathways in Maintaining Intestinal Homeostasis [3 DPI colon] 35 samples shares ripk1, maintaining, homeostasis
GSE245632 Generation and characterization of mature hepatocyte organoids for liver metabolic studies 45 samples shares hepatocyte, liver
GSE238201 Loss of PPARα function promotes epigenetic dysregulation of lipid homeostasis driving ferroptosis and pyroptosis lipotoxicity in Metabolic Dysfunction Associated Steatotic Liver Disease (MASLD) [RNA-seq] 12 samples shares homeostasis, liver
GSE228579 Chronic stress disrupts hepatic homeostasis and accelerates liver cancer progression through ADRB2 signaling 66 samples shares homeostasis, liver
GSE285614 Adipocyte death drives fat redistribution from adipocytes to hepatocytes in steatotic liver disease via S100A8+ macrophages [RNA-seq] 34 samples shares hepatocyte, liver
GSE294857 Distinct hepatocyte adaptations during gestation and lactation [pregnant_liver_mm10] 31 samples shares hepatocyte, liver
GSE324743 Hepatocytes promote liver metastasis of pancreatic cancer by providing exogenous serine 24 samples shares hepatocyte, liver

Search all mouse RNA-seq datasets in GEO →

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