GEO series

Muscle fiber Myc is dispensable for muscle growth and its forced expression severely perturbs homeostasis [Akt_MycKO]

GSE287997 Mus musculus (mouse) RNA-seq (gene expression) 23 samples Submitted 2025/02/27 Platform GPL24247

At a glance

RNA-seq (gene expression) · mouse · 23 samples

What it is: RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism: Mus musculus (mouse)
Samples: 23 samples: 6 conditions, mostly with about 4 replicates eachGroups are read from the sample names, not from the paper. Check the list below.
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper: Muscle fiber Myc is dispensable for muscle growth and its forced expression severely perturbs homeostasisNature communications · 2025

Summary (written by the submitters)

The oncogenic transcription factor Myc stimulates many growth processes including cell cycle progression and ribosome biogenesis. Myc expression is low in adult skeletal muscle, but is upregulated upon growth stimuli. Furthermore, muscle fiber Myc overexpression recapitulates many aspects of growth-related gene expression, leading to the hypothesis that Myc mediates pro-growth responses to anabolic stimuli, such as exercise.

Read the full summary ↓

Here, we tested this hypothesis by examining mouse models in which Myc was specifically eliminated or overexpressed in skeletal muscle fibers or muscle stem cells (MuSC). While muscle fiber Myc expression increased during muscle growth and Myc expression in MuSCs was required for successful muscle regeneration, muscle fiber Myc expression was dispensable for post-natal, mechanical overload or PKBα/Akt1-induced muscle growth in mice. Similarly, constitutive Myc expression did not promote skeletal muscle hypertrophy, but instead impaired muscle fiber structure and function within days. These data question the role of Myc in skeletal muscle growth.

Published in

Muscle fiber Myc is dispensable for muscle growth and its forced expression severely perturbs homeostasis

Ham DJ, Semeraro M, Berger BM et al. · Nature communications 2025 · PMID 40180928 · doi:10.1038/s41467-025-58542-7

This dataset

Open GSE287997 in finder → Find more mouse RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE287997_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1215425 and SRA study SRP559743. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 40180928) ↗

Study design

6 conditions, mostly with about 4 replicates each

CON_3d ×4 MycKO_3d ×4 AKT_3d ×4 AKT_14d ×4 AKT-MycKO_14d ×4 AKT-MycKO_3d ×3

Supports a between-group comparison across 23 samples.

How we read this: 6 replicated groups read from 23 sample titles; they account for 23 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

GSM8757236 CON_3d_35541 CON_3d
GSM8757237 CON_3d_35544 CON_3d
GSM8757238 CON_3d_36114 CON_3d
GSM8757239 CON_3d_36116 CON_3d
GSM8757240 MycKO_3d_35540 MycKO_3d
GSM8757241 MycKO_3d_35543 MycKO_3d
GSM8757242 MycKO_3d_36321 MycKO_3d
GSM8757243 MycKO_3d_36750 MycKO_3d
GSM8757244 AKT_3d_35539 AKT_3d
GSM8757245 AKT_3d_35542 AKT_3d
GSM8757246 AKT_3d_35546 AKT_3d
GSM8757247 AKT_3d_36751 AKT_3d
GSM8757248 AKT-MycKO_3d_35545 AKT-MycKO_3d
GSM8757249 AKT-MycKO_3d_36319 AKT-MycKO_3d
GSM8757250 AKT-MycKO_3d_36322 AKT-MycKO_3d
GSM8757251 AKT_14d_34305 AKT_14d
GSM8757252 AKT_14d_34910 AKT_14d
GSM8757253 AKT_14d_35118 AKT_14d
GSM8757254 AKT_14d_35228 AKT_14d
GSM8757255 AKT-MycKO_14d_34173 AKT-MycKO_14d
GSM8757256 AKT-MycKO_14d_34306 AKT-MycKO_14d
GSM8757257 AKT-MycKO_14d_34404 AKT-MycKO_14d
GSM8757258 AKT-MycKO_14d_34908 AKT-MycKO_14d

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.