← BioTransfer GEO Dataset Finder
GEO series

RNA sequencing of HUVEC, HCMEC and human primary skeletal muscle cells in response to in vitro mechanical loading

GSE288064 Homo sapiens (human) RNA-seq (gene expression) 36 samples Submitted 2026/07/10 Platform GPL24676
At a glance

RNA-seq (gene expression) · human · 36 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Homo sapiens (human)
Samples
36 samples: 6 conditions, mostly with about 6 replicates eachGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Summary (written by the submitters)
Our objective was to profile and compare the transcriptomes of mechanically loaded vs. static cells in three different human cell lines: Human umbilical vein endothelial cells (HUVEC), Human cardiac microendothelial cells (HCMEC) and primary human skeletal muscle cells (HSkM). Cells were mechanically loaded for 5h and total RNA was collected immediately after. The mechanical loading experiment was conducted for all three cell lines separately, with 6 biological replicates in the experimental group and 6 replicates as static controls, resulting in a total of 12 samples per cell line.
This dataset
Open GSE288064 in finder → Find more human RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE288064_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1215982 and SRA study SRP560018. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗
Study design
6 conditions, mostly with about 6 replicates each
HUVEC, static control, ×6 HUVEC, mechanical stretch, ×6 HCMEC, static control, ×6 HCMEC, mechanical stretch, ×6 HSkM, static control, ×6 HSkM, mechanical stretch, ×6

Supports a between-group comparison across 36 samples.

How we read this: 6 replicated groups read from 36 sample titles; they account for 36 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

Search all human RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.