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Eosinophils promote a second wave of postnatal smooth muscle cell differentiation in intestinal villi

GSE289872 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2026/05/12 Platform GPL17021
Summary
Intestinal villus smooth muscle cells (SMCs) play a critical structural and functional role in the gut, notably facilitating dietary fat transport via villus-associated lymphatic lacteals. In the early postnatal period subepithelial PDGFRα+ fibroblasts are guided by lacteals to differentiate and assemble into villus SMCs. We show now that villus SMC expansion can occur in the absence of lacteals suggesting the involvement of additional mechanisms. Using whole-mount imaging, genetic lineage tracing, and eosinophil-deficient models, we identify a second wave of intestinal SMC differentiation where villus tip perivascular PDGFRβ+ cells are a reservoir of distal SMC progenitors. We demonstrate that resident intestinal eosinophils act as key drivers and coordinators of TGFβ-dependent villus SMC expansion and maturation. Our findings propose a model in which distinct mesenchymal sources provide progenitors for generation of postnatal villus SMCs and reveal a novel morphogenetic role of intestinal eosinophils as critical mediators of SMC development in the gut.
Published in
Eosinophils promote a second wave of postnatal smooth muscle differentiation in intestinal villi
Petrova TV, de Korodi K, Berg T et al. · The Journal of cell biology 2026 · PMID 42118023 · doi:10.1083/jcb.202505012
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Also filed as BioProject PRJNA1225230 and SRA study SRP564651. Searching any of these in the dataset finder brings you back here.

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