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Shaking culture attenuates circadian rhythms in induced pluripotent stem cells during osteogenic differentiation through the YAP-TEAD axis

GSE291628 Mus musculus Expression profiling by high throughput sequencing 4 samples Submitted 2025/03/14 Platform GPL24247
Summary
Circadian rhythms synchronize cellular activities with the Earth’s 24-hour cycle, primarily regulated by clock genes that influence metabolism, physiology, and osteogenesis. While genetic regulation of circadian rhythms is well characterized, how extracellular mechanical forces modulate these rhythms during iPSC osteogenic differentiation remains unclear. Here, we show that shaking culture, which promotes three-dimensional iPSC-EB formation, disrupts clock gene oscillations by activating YAP-TEAD signaling. Inhibition of YAP-TEAD with verteporfin restores circadian oscillations and enhances osteogenesis, highlighting a biomechanical-circadian interplay with implications for regenerative medicine.
Published in
Shaking culture attenuates circadian rhythms in induced pluripotent stem cells during osteogenic differentiation through the TEAD-Fbxl3-CRY axis
Fu Y, Okawa H, Vinaikosol N et al. · Cell death discovery 2025 · PMID 40413171 · doi:10.1038/s41420-025-02533-6
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Also filed as BioProject PRJNA1234546 and SRA study SRP569580. Searching any of these in the dataset finder brings you back here.

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