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Intestinal mast cell-derived leukotrienes mediate the anaphylactic response to ingested antigens

GSE293904 Mus musculus Expression profiling by high throughput sequencing 4 samples Submitted 2025/08/07 Platform GPL24247
Summary
Anaphylaxis is a life-threatening complication of allergen exposure. While mechanisms governing anaphylaxis after intravenous injection have been defined in mice, these models neglect mucosal exposure that accompanies food ingestion. We investigated the role of mast cell populations within the intestine of mice in response to antigens delivered orally. Oral anaphylaxis required IgE-FcεR1 signaling, and profiling of intestinal mast cells revealed a rapidly developing population shaped by epithelial cues. Intestinal mast cells were largely epithelium-resident and displayed divergent transcriptomes and effector functions from connective tissue mast cells found throughout the body. Histamine synthesis was diminished and leukotriene generation enhanced. Mice genetically deficient in cysteinyl leukotriene synthesis, or those treated with aLOX5 antagonist, Zileuton, were protected from oral anaphylaxis whereas that elicited by intravenous injection was unaltered.
Published in
Intestinal mast cell-derived leukotrienes mediate the anaphylactic response to ingested antigens
Bachtel ND, Cullen JL, Liu M et al. · Science (New York, N.Y.) 2025 · PMID 40773543 · doi:10.1126/science.adp0246
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Also filed as BioProject PRJNA1247403 and SRA study SRP577066. Searching any of these in the dataset finder brings you back here.

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