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Identification of fibroblast-derived sFRP2 as a new therapeutic target and engineering of siRNA therapies for uterine scarring

GSE293910 Homo sapiens (human) RNA-seq (gene expression) 12 samples Submitted 2025/07/06 Platform GPL24676Platform GPL24247
At a glance

RNA-seq (gene expression) · human · 12 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Homo sapiens (human)
Samples
12 samples: 3 × Homo sapiens, uterine myometrial tissue, control, biol vs 3 × Homo sapiens, uterine myometrial tissue, Scar uterus disease group, biol vs 3 × Mus musculus, uterine myometrial tissue, control, biol vs 3 × Mus musculus, uterine myometrial tissue, Scar uterus disease group, biolGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Identifying fibroblast-derived sFRP2 as a therapeutic target and engineering siRNA therapy for uterine scarringNature communications · 2025
Summary (written by the submitters)
Uterine scarring (US) is a common complication following uterine injury, characterized by abnormal wound healing in the endometrial or myometrial tissue. However, the underlying mechanisms contributing to scar formation remain unclear, impeding the development of effective drugs for US. Here, we identified that secreted frizzled-related protein 2 (sFRP2) is highly expressed in human and mouse uterine tissues with US, particularly in uterine fibroblasts (UFIBs).
Read the full summary ↓
Using mouse models, we demonstrated that sFRP2 overexpression in the healthy uterus induced US features and exacerbated surgery-induced scarring, while sFRP2 knockout inhibited US development and scarring-transformation in UFIBs, highlighting the critical role of sFRP2 in mediating US. Mechanistically, sFRP2 promotes uterine scar formation by activating the non-canonical Wnt signaling pathway and calcium influx in UFIBs. Additionally, therapeutic potential of targeting sFRP2 was demonstrated by developing siRNA against sFRP2 and engineering delivery systems of lipid nanoparticles and injectable hydrogel. These siRNA therapies effectively inhibited sFRP2 expression, alleviated uterine scar formation, and improved pregnancy outcomes in mice, underscoring the great potential of sFRP2 as a promising therapeutic target for US.
Published in
Identifying fibroblast-derived sFRP2 as a therapeutic target and engineering siRNA therapy for uterine scarring
Cheng J, Zhang S, Gui Q et al. · Nature communications 2025 · PMID 40715133 · doi:10.1038/s41467-025-62248-1
This dataset
Open GSE293910 in finder → Find more RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE293910_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1247411. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 40715133) ↗
Study design
3 × Homo sapiens, uterine myometrial tissue, control, biol vs 3 × Homo sapiens, uterine myometrial tissue, Scar uterus disease group, biol vs 3 × Mus musculus, uterine myometrial tissue, control, biol vs 3 × Mus musculus, uterine myometrial tissue, Scar uterus disease group, biol

Supports a case/control comparison: 6 samples read as cases, 6 as controls.

How we read this: 4 replicated groups read from 12 sample titles; they account for 12 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
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