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RNA-seq analysis of non-vascular smooth muscle cells (NVSMCs) isolated from the lung, intestine and bladder

GSE301480 Mus musculus Expression profiling by high throughput sequencing 8 samples Submitted 2026/04/11 Platform GPL30172
Summary
None of the currently available Cre recombinase-expressing mouse lines allows for exclusive and specific manipulation of non-vascular smooth muscle cells (NVSMCs). To address this, we focused on the Chrm2 gene, which encodes the M2 muscarinic acetylcholine receptor (M2R), a G protein-coupled receptor (GPCR) previously reported to be expressed selectively in NVSMCs and also in CD45-positive immune cells. In contrast, Acta2 is broadly and strongly expressed in all smooth muscle cells (SMCs), including both vascular and non-vascular populations. To achieve specific labeling of NVSMCs, we generated NVSMC-effector mice by combining Chrm2-Dre with Acta2-Rox-CreER and R26-LoxP-GFP alleles. Following tamoxifen administration, we observed robust GFP fluorescence specifically in NVSMCs of the lung, stomach, and intestine. Notably, GFP labeling was virtually absent in vascular SMCs across multiple organs, confirming the specificity of this genetic strategy.
Published in
Cell Type-Specific Targeting of Different Smooth Muscle Cell Populations by Intersectional Genetics
Wang L, Staps M, Günther S et al. · Circulation 2026 · PMID 41969103 · doi:10.1161/CIRCULATIONAHA.125.076318
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Also filed as BioProject PRJNA1285311 and SRA study SRP597315. Searching any of these in the dataset finder brings you back here.

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