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Effect of cardiomyocyte-specific activation of nuclear receptor subfamily 4 group A member 2 (NR4A2) on poly(A) RNA expression profile in the mouse heart [RNA-seq]

GSE294805 Mus musculus (mouse) RNA-seq (gene expression) 32 samples Submitted 2026/02/18 Platform GPL24247
At a glance

RNA-seq (gene expression) · mouse · 32 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
32 samples: 8 conditions, mostly with about 4 replicates eachGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Nuclear receptor subfamily 4 group a member 2 induces a Warburg-like effect and promotes phospholipids synthesis in the mouse heartPhysiological genomics · 2026
Summary (written by the submitters)
Nuclear receptor subfamily 4 group A member 2 (NR4A2) is a widely expressed immediate early gene induced by a host of physiological signals such as growth factors, cytokines, and neurotransmitters. In the adult mammalian heart, including in cardiomyocytes, NR4A2 mRNAs increase more than 60-fold in response to beta-adrenergic stimulation.
Read the full summary ↓
However, the nature of the transcriptional remodeling induced by NR4A2 in the stressed heart has remained poorly investigated. We show here that NR4A2 activation in mouse cardiomyocytes profoundly alters the sub-transcriptomes controlling metabolic pathways and calcium transients in the heart. At the metabolic level, NR4A2 induced the expression of glycolytic enzymes while simultaneously down-regulating fatty acid oxidation. Increased NR4A2 expression was also associated with the repression of several genes critical for t-tubules formation, dyads organization, and calcium flux. The findings shed light on a novel regulatory mechanism controlling cardiac homeostasis, and establish a framework to investigate further the roles played by NR4A2 in fetal reprogramming of the adult mammalian heart under various stress conditions.
Published in
Nuclear receptor subfamily 4 group a member 2 induces a Warburg-like effect and promotes phospholipids synthesis in the mouse heart
Ashraf S, Odogwu D, McPherson DD et al. · Physiological genomics 2026 · PMID 41801102 · doi:10.1152/physiolgenomics.00319.2025
This dataset
Open GSE294805 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE294805_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1251628 and SRA study SRP579168. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41801102) ↗
Study design
8 conditions, mostly with about 4 replicates each
Heart ventricles, Nr4a2-icTg female 1, Day ×4 Heart ventricles, Nr4a2-icTg female 2, Day ×4 Heart ventricles, Nr4a2-icTg male 1, Day ×4 Heart ventricles, Nr4a2-icTg male 2, Day ×4 Heart ventricles, Cre control female 1, Day ×2 Heart ventricles, Cre control female 2, Day ×2 Heart ventricles, Cre control male 1, Day ×2 Heart ventricles, Cre control male 2, Day ×2 +8 more

Supports a between-group comparison across 24 samples.

How we read this: 8 replicated groups read from 32 sample titles; they account for 24 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

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