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Transcriptome profiling of B16F10 cells and differentiated B16F10 cells induced by forced expresssion of Myod1, Pparg, or by knockdown of Setdb1 using RNA-sequencing.

GSE295152 Mus musculus Expression profiling by high throughput sequencing 5 samples Submitted 2026/06/01 Platform GPL24247
Summary
The core proeprty of cancer cells is neural stemness, which determines tumorigenicity and pluripotency. Therefore, cancer cells can supposedly be induced to differentiate along different lineages. B16F10 is a mouse melanoma cell line. We found that B16F10 cells differentiated into muscle cells or adipocytes upon induction by muscle or adipocyte differentiation factor Myod1 or Pparg, respectively. Moreover, Setdb1 is an epigenetic modification factor responsible for catalyzing the histone modification H3K9me3. Transcription of Setdb1 gene is enriched in embryonic neural cells during vertebrate embryogenesis. Setdb1 is upregulated in cancer cells and promotes cancers. We found that knockdown of Setdb1 in B16F10 cells led to a neuronal-like differentiation phenotype.
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Direct links to NCBI, no account and no request form: the whole study as GSE295152_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 5 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1253643 and SRA study SRP580254. Searching any of these in the dataset finder brings you back here.

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