RNA-seq data of Hepatic Insulin Response in Young and Aged Mice

To investigate age-related differences in hepatic insulin response at the transcriptomic level, we profiled RNA sequencing in liver tissue from young (6-month-old) and old (20-month-old) male C57BL/6J mice following a hyperinsulinemic-euglycemic clamp. Mice underwent detailed metabolic phenotyping, including body composition analysis and indirect calorimetry, followed by surgical catheterization and a standardized insulin clamp procedure with stable isotope tracer infusions. Liver tissue was collected immediately after the clamp, and RNA was extracted. The tracer studies enabled quantification of hepatic fluxes through pyruvate dehydrogenase, fatty acid oxidation, citrate synthase, and pyruvate carboxylase.