APOE4 expression causes distinct effects on meningeal immunity, brain lipid composition, and cognition in male and female mice

This experiment was designed to understand the distinct effects of sex and APOE genotype on the transcriptome of meningeal dural vascular and resident immune cells. After transcardiac perfusion with a buffered saline solution containing heparin and actinomycin D, meningeal dural whole mounts were immediately dissected from the skulls of 12-month-old male and female mice expressing APOE3 or APOE4 and digested into a single-cell suspension. After enrichment for CD31+ cells via positive selection using MACS, the cells were counted and approximately 5000 cells per sample were used to obtain single-cell gene expression libraries using 10x Genomics equipment and reagents, namely the Chromium Single Cell 3' Reagent Kits. The generated libraries were sequenced in 2 lanes of a NovaSeq 6000 S4 PE100 flow cell.