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Single cell expression profiling of brain tumors in mice intracranially injected with 2x105 glioblastoma tumor cells isolated from NTv-a;hUBC-CreERT2;Adar1flox/flox mice and treated with tamoxifen to induce Adar1 deletion, or with vehicle control.

GSE295661 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2025/09/02 Platform GPL24247
Summary
Glioblastomas are the most frequent and aggressive primary brain tumors. Current treatments invariably fail, a consequence of the pronounced heterogeneity and plasticity of glioblastoma cells, as well as the contribution of an immunosuppressive microenvironment that promotes tumor progression and resistance to therapy. Here, we exploited an innate immunity checkpoint, RNA sensing, to simultaneously target cancer cells and their supporting microenvironment. Using various immunocompetent mouse models of glioblastoma, we found that genetic deletion of Adar1, a key regulator of the RNA sensing pathway, resulted in significantly reduced tumor growth and prolonged survival. Mechanistically, these effects were mediated by two processes: cancer-cell intrinsic responses and reprogramming of the immune microenvironment that fostered a pro-inflammatory, anti-tumoral state via type I interferon signaling. These findings establish proof of concept for the therapeutic potential of targeting ADAR1 in glioblastoma, offering new strategies for the treatment of this aggressive disease.
Published in
Cancer cell and microenvironmental rewiring by ADAR1 loss impairs glioblastoma tumor growth and extends survival
Álvarez-Prado ÁF, Hernández-Barranco A, Rentsch L et al. · Cell reports 2025 · PMID 40892541 · doi:10.1016/j.celrep.2025.116151
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Direct links to NCBI, no account and no request form: the whole study as GSE295661_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1255552 and SRA study SRP585311. Searching any of these in the dataset finder brings you back here.

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