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Inducing bacterial calcification for systematic treatment and immunomodulation against Methicillin-resistant Staphylococcus aureus

GSE298599 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2025/05/30 Platform GPL34290
Summary
Methicillin-resistant Staphylococcus aureus (MRSA) has become one of the deadliest bacteria globally due to antibiotic resistance. In this study, we crosslinked antigen-binding fragments of monoclonal antibodies against the wall-teichoic acid of S. aureus with polysialic acid (PSA) to form an antibody‒PSA conjugate (APC), which can effectively target and induce calcification on the surface of MRSA. This process eliminates bacteria by hindering the energy metabolism and multiple essential metabolic pathways of MRSA. We find thatbacterial calcification leads to increased expression of calprotectin, S100A8/S100A9, in macrophages and monocytes in vivo and can stimulate the activation of macrophages to an inflammatory state, thereby promoting bacterial eradication as an immunomodulator. Systemic administration of APC demonstrates high efficacy and safety for treating chronic lung infections and chronic osteomyelitis caused by MRSA in mice. This study offers a promising therapy for treating drug-resistant bacteria and related refractory pathogenic infections.
Published in
Inducing bacterial calcification for systematic treatment and immunomodulation against methicillin-resistant Staphylococcus aureus
Zhang W, Liu L, Zhang Q et al. · Nature biotechnology 2026 · PMID 40664980 · doi:10.1038/s41587-025-02736-3
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Also filed as BioProject PRJNA1269999 and SRA study SRP588883. Searching any of these in the dataset finder brings you back here.

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