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Elevated expression of the molecular clock suppressors Period 1 and Period 2 promote myotube atrophy in vitro with implications to the loss of limb muscle mass following androgen deprivation

GSE299632 Mus musculus (mouse) RNA-seq (gene expression) 20 samples Submitted 2025/08/06 Platform GPL34290
At a glance

RNA-seq (gene expression) · mouse · 20 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
20 samples: 10 × C2C12 cells, Gfp transduction, vs 5 × C2C12 cells, Per2 transduction, vs 5 × C2C12 cells, Per1 transduction,Groups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Sustained Accumulation of Molecular Clock Suppressors Period 1 and Period 2 Promotes C2C12 Myotube Atrophy Through an Autocrine-Mediated Mechanism With Relevance to Androgen Deprivation-Induced Limb Muscle Mass LossFunction (Oxford, England) · 2025
Summary (written by the submitters)
Low testosterone levels in men (hypogonadism) are associated with loss of limb muscle mass. We previously showed molecular clock function is disrupted in limb muscle by androgen deprivation. That clock disruption coincided with higher protein levels of the molecular clock suppressor, Period 2 (Per2). The primary purpose of this study was to assess whether Per2 regulates muscle size.
Read the full summary ↓
Transducing fully differentiated C2C12 myotubes with adenovirus overexpressing Per2 was sufficient to lower myotube diameter. Moreover, deletion of Per2 in male mice partially maintained mass of the tibialis anterior (TA) muscle following castration-induced androgen deprivation. The partial maintenance of mass was specific to the TA even though molecular clock disruption is evident in other limb muscles. Thus, the secondary purpose of this study was to assess whether Per1 also regulates muscle size. Overexpression of the other primary molecular clock suppressor, Per1, in C2C12 myotubes was also sufficient to decrease myotube diameter. The lower myotube diameter in response to Per1 or Per2 overexpression was due in part to an autocrine-mediated inflammatory signals as conditioned media from Per1 or Per2 overexpressing myotubes contained elevated cytokines/chemokines, and the Per conditioned media was sufficient to lower C2C12 myotube diameter. The mRNA contents of inflammatory genes altered following Per1 or Per2 overexpression in vitro were altered in limb muscle in vivo following castration. These data define a novel muscle mass/size regulatory function of the primary molecular clock suppressors with direct implications for their role in regulating limb muscle mass loss in response to low androgen levels
Published in
Sustained Accumulation of Molecular Clock Suppressors Period 1 and Period 2 Promotes C2C12 Myotube Atrophy Through an Autocrine-Mediated Mechanism With Relevance to Androgen Deprivation-Induced Limb Muscle Mass Loss
Laskin GR, Steiner JL, Ayers-Creech WA et al. · Function (Oxford, England) 2025 · PMID 40632504 · doi:10.1093/function/zqaf030
This dataset
Open GSE299632 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE299632_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1275803 and SRA study SRP592065. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 40632504) ↗
Study design
10 × C2C12 cells, Gfp transduction, vs 5 × C2C12 cells, Per2 transduction, vs 5 × C2C12 cells, Per1 transduction,

Supports a between-group comparison across 20 samples.

How we read this: 3 replicated groups read from 20 sample titles; they account for 20 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
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