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Rbm24 promotes outer hair cell survival through Insm1 repression while independently regulating hair bundle morphogenesis

GSE300428 Mus musculus (mouse) RNA-seq (gene expression) 163 samples Submitted 2026/03/28 Platform GPL34290
At a glance

RNA-seq (gene expression) · mouse · 163 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
163 samples: 56 × P7_Exp_OHC vs 43 × P7_Ctrl_OHC vs 38 × P7_Ctrl_IHC vs 26 × P7_Exp_IHCGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Rbm24 promotes outer hair cell survival through Insm1 repression while independently regulating hair bundle morphogenesisDevelopment (Cambridge, England) · 2026
Summary (written by the submitters)
The inactivation of Rbm24, an RNA-binding protein, leads to cell death and hair bundle defects in cochlear outer hair cells (OHCs). However, the underlying molecular mechanisms remain unclear. To address this, we have performed comprehensive transcriptomic profiling of purified wild type and Rbm24-/- OHCs at postnatal day 7 (P7). Loss of Rbm24 perturbs numerous genes associated with hair bundle morphogenesis and delays the overall OHC differentiation program.
Read the full summary ↓
Insm1, a key transcription factor normally downregulated by P2, remains aberrantly and persistently expressed in Rbm24-/- OHCs. Overexpression of Insm1 alone induces OHC death, whereas simultaneous inactivation of Rbm24 and Insm1 largely rescues OHC survival but only partially restores hair bundle morphology. It demonstrates that Rbm24 promotes OHC survival independently of its role in regulating hair bundle morphogenesis. Collectively, our findings establish Rbm24 as a dual function regulator that ensures OHC survival by acting as a critical repressor of Insm1 expression, while independently orchestrating hair bundle assembly. These results highlight the central role of Rbm24 in coordinating OHC differentiation and structural maturation and provide insights into potential molecular targets for hair cell regeneration.
Published in
Rbm24 promotes outer hair cell survival through Insm1 repression while independently regulating hair bundle morphogenesis
Li C, Zhao Y, Wang L et al. · Development (Cambridge, England) 2026 · PMID 41884985 · doi:10.1242/dev.204234
This dataset
Open GSE300428 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE300428_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1280943 and SRA study SRP594114. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41884985) ↗
Study design
56 × P7_Exp_OHC vs 43 × P7_Ctrl_OHC vs 38 × P7_Ctrl_IHC vs 26 × P7_Exp_IHC

Supports a between-group comparison across 163 samples.

How we read this: 4 replicated groups read from 163 sample titles; they account for 163 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

+ 123 more — browse all 163 samples with per-sample file links →

Datasets on a similar topic

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