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Effects of Histidine restriction in Jurkat cells in vitro

GSE300867 Homo sapiens (human) RNA-seq (gene expression) 25 samples Submitted 2026/02/26 Platform GPL21290
At a glance

RNA-seq (gene expression) · human · 25 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Homo sapiens (human)
Samples
25 samples: 5 conditions, mostly in triplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
A dietary pan-amino acid dropout screen in vivo reveals a critical role for histidine in T-ALLbioRxiv : the preprint server for biology · 2025
Summary (written by the submitters)
Here we report the gene expression profile of T-ALL cell line - Jurkat, cultured in different levels of Histidine restricted media and collected at two time points - Day 3 and Day 6
Published in
A dietary pan-amino acid dropout screen in vivo reveals a critical role for histidine in T-ALL
Mandleywala K, Ulrich S, da Silva-Diz V et al. · bioRxiv : the preprint server for biology 2025 · PMID 41509383 · doi:10.64898/2025.12.21.694897
This dataset
Open GSE300867 in finder → Find more human RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE300867_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1282482 and SRA study SRP594754. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41509383) ↗
Study design
5 conditions, mostly in triplicate
50His ×3 25His ×3 12-5His ×3 NoHis ×3 100His ×2 100His-1-D ×1 100His-2-D ×1 100His-3-D ×1 +8 more

Supports a between-group comparison across 14 samples.

How we read this: 5 replicated groups read from 25 sample titles; they account for 14 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

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