Effects of 7 EAA restriction (Histidine, Methionine, Valine, Leucine, Isoleucine, Phenylalanine and Tryptophan) in Jurkat cells in vitro
RNA-seq (gene expression) · human · 59 samples
- What it is
- RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
- Organism
- Homo sapiens (human)
- Samples
- 59 samples
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- A dietary pan-amino acid dropout screen in vivo reveals a critical role for histidine in T-ALLbioRxiv : the preprint server for biology · 2025
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE301669_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.
Also filed as BioProject PRJNA1285951 and SRA study SRP598144. Searching any of these in the dataset finder brings you back here.
The sample list for this study is not cached yet. Press Load samples and it will be fetched from NCBI.
+ 59 more — browse all 59 samples with per-sample file links →
- GSE301665 Effects of Histidine restriction in DND-41 cells in vitro 25 samples shares restriction, histidine
- GSE300867 Effects of Histidine restriction in Jurkat cells in vitro 25 samples shares restriction, histidine
- GSE270333 Methionine Restriction Reduces Lung Cancer Progression and Increases Chemotherapy Response 12 samples shares restriction, methionine
- GSE234806 Effect of methionine restriction on gene expression of non-KMT2A-rearranged NALM-6 and KMT2A-rearranged SEM BCP-ALL cell lines 12 samples shares restriction, methionine
- GSE72131 Genome-wide map of H3K4me3 before and after methionine restriction in HCT116 cells. 12 samples shares restriction, methionine
- GSE55285 Expression data from undifferentiated human ES cell line, khES3, grown using complete, methionine, leucine or lysine deprived media and ES cell derived endoderm 9 samples shares methionine, leucine
- GSE241864 The SLC1A1/EAAT3 Dicarboxylic Amino Acid Transporter is an Epigenetically Dysregulated Nutrient Carrier that Sustains Oncogenic Metabolic Programs 8 samples shares eaa
- GSE263838 The interactive effect of sustained sleep restriction and resistance exercise on skeletal muscle transcriptomics in young females 38 samples shares restriction
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.