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The impact of Hnrnpl deficiency on transcriptional patterns of developing muscle cells   

GSE302000 Mus musculus Expression profiling by high throughput sequencing 4 samples Submitted 2025/09/08 Platform GPL28599
Summary
Heterogeneous nuclear ribonucleoproteins (hnRNPs) bind to RNA, regulating gene expression and splicing. HnRNP L contributes to muscle development and the pathogenesis of myotonic dystrophy. We hypothesized that hnRNP L regulates muscle expression and splicing patterns. Using nanopore long read transcriptome sequencing and qPCR analyses, we investigated the impact of Hnrnpl knockdown on myoblasts and knockdown of the orthologous gene smooth in Drosophila. Notch signaling genes and muscle-related genes were dysregulated in both models. Several genes had altered splicing patterns, including Lamp2, Fhl1, and Dtna. Our findings indicate that hnRNP L regulates muscle gene transcription. We demonstrate the capabilities of long read transcriptome sequencing to study muscle development. Future studies could examine therapeutic implications of hnRNP L regulation for muscle diseases.
Published in
The impact of Hnrnpl deficiency on transcriptional patterns of developing muscle cells
Littel HR, Gunasekaran M, Daugherty AL et al. · FEBS open bio 2026 · PMID 40944391 · doi:10.1002/2211-5463.70117
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Also filed as BioProject PRJNA1288256 and SRA study SRP599044. Searching any of these in the dataset finder brings you back here.

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