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Mitochondrial ROS induces metabolic adaptations and maladaptations that differentially regulate ferroptosis sensitivity of the hepatocytes

GSE302789 Mus musculus (mouse) RNA-seq (gene expression) 12 samples Submitted 2026/07/26 Platform GPL24247
At a glance

RNA-seq (gene expression) · mouse · 12 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
12 samples: 3 × Mouse hepatocytes; control vs 3 × Mouse hepatocytes; FAC treated vs 3 × Mouse hepatocytes; liproxstatin-1 + FAC treated vs 3 × Mouse hepatocytes; Mito-TEMPO + FAC treatedGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Summary (written by the submitters)
Ferroptosis mediates the pathogenesis of a plethora of diseases. Mitochondrial reactive oxygen species (mtROS) plays a critical role in ferroptosis induction, but the precise mechanism by which mtROS promotes lipid peroxidation-dependent ferroptosis remains poorly understood. Herein, we employed hepatocytes and iron-induced ferroptosis as models to identify the specific mtROS-mediated redox and metabolic changes that modulate ferroptosis sensitivity.
Read the full summary ↓
Iron overload induced the activation of multiple antioxidative mechanisms, including altered transport and metabolism of redox-active metals, small Maf (sMaf)-mediated activation of transcription factor nuclear factor erythroid 2-related factor 2 (NRF2), and an increased potential of glutathione biosynthesis. Iron also induced mtROS-dependent suppression of fatty acid oxidation, the activities of citric acid cycle and mitochondrial respiration, protecting against ferroptosis. We further identified that mtROS signaling potentially impairs the biosynthesis of coenzyme Q10 (CoQ) by attenuating the expression of genes in the mevalonate pathway and CoQ8A, a stabilizer of CoQ complex. Importantly, elevating CoQ8A expression mitigated, whereas silencing CoQ8A expression enhanced, the vulnerability of hepatocytes to ferroptosis. The mtROS-mediated downregulation of CoQ8A was dependent on farnesoid X receptor (FXR) and retinoid X receptors (RXRs). Our study demonstrated, for the first time, that mtROS induces impaired CoQ biosynthesis as a maladaptive change that promotes ferroptosis.
This dataset
Open GSE302789 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE302789_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1292175 and SRA study SRP601217. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗
Study design
3 × Mouse hepatocytes; control vs 3 × Mouse hepatocytes; FAC treated vs 3 × Mouse hepatocytes; liproxstatin-1 + FAC treated vs 3 × Mouse hepatocytes; Mito-TEMPO + FAC treated

Supports a case/control comparison: 9 samples read as cases, 3 as controls.

How we read this: 4 replicated groups read from 12 sample titles; they account for 12 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

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